Christoph Mark

Christoph Mark

Christoph Mark

 

PhD student

Room 02.076

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Conference presentations

 

Dynamics and heterogeneity in tumor cell migration - How do cells move? (Talk & Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Ben Fabry

RTG1962 Membrane Winterworkshop, Pitztal, Austria (2014)

 

Dynamics and heterogeneity in tumor cell migration (Talk & Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Lena Lautscham, Ben Fabry

5th Annual Symposium - Physics of Cancer, Leipzig, Germany (2014)

 

Uncovering the time dependent statistics of migrating tumor cells (Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Lena Lautscham, Christoph Kämmerer, Ben Fabry

DPG-Frühjahrstagung der Sektion Kondensierte Materie, Dresden, Germany (2014)

Publications Graeme Whyte

 

Optofluidic rotation of living cells for single-cell tomography

 

gwhyte-pub-29-flowrot

 

T Kolb, S Albert, M Haug, G Whyte
Journal of Biophotonics accepted (2014)

 

Dynamic Fire Optic Spanner

 

gwhyte-DFOS-scheme

 

 

T Kolb, S Albert, M Haug, G Whyte
Lab on a Chip in press (2014)

 

Impact of heating on passive and active biomechanics of suspended cells

  gwhyte-pub-27-heating
  

 

CJ Chan, G Whyte, L Boyde, G Salbreux, J Guck
Interface Focus
accepted (2014)

 

Viscoelastic properties of differentiating cells are fate- and function-dependent

  gwhyte-pub-24-HL60-PLOSOne-2012 

 

A Ekpenyong, G Whyte, K Chalut, F Lautenschlaeger, C Fiddler, D Olin, E Chilvers, M Beil, J Guck,
PLoS ONE 7(9) (2012)

 

Mechanical environment modulates biological properties of oligodendrocyte progenitors cells

 gwhyte-pub-23-OPC-StemCellDev-2012

 

A Jagielska, A Norman, G Whyte, KJ Van Vliet, J Guck, RJM Franklin
Stem Cells and Development 21 (2012)

 

Validation and perspectives of a monolithic optical stretcher fabricated by femtosecond lasers

  gwhyte-pub-26-fsChip-BioMedOptEx-2012  

 

N Bellini, F Bragheri, R Ramponi, I Cristiani, J Guck, R Osellame, G Whyte
BioMed Opt Exp,
3, (10), 2658 (2012)

 

Comparison of Stresses on Homogeneous Spheroids in the Optical Stretcher Computed with Geometrical Optics and Generalised Lorenz-Mie Theory

gwhyte-pub-25-ComparisonStresses-AppOpt-2012  

 

L Boyde, A Ekpenyong, G Whyte, J Guck,
Applied Optics 51 (2012)

 

Generation of picoliter droplets with defined contents and concentration gradients from the separation of chemical mixtures

 gwhyte-pub-22-HPLC-AnalChem-2010
AB Theberge, G Whyte and WTS Huck
Anal Chem, 82 3449 (2010)

 

Suzuki-Miyaura coupling reactions in aqueous microdroplets with catalytically active fluorous interfaces

 Graphical abstract: Suzuki–Miyaura coupling reactions in aqueous microdroplets with catalytically active fluorous interfaces

AB Theberge, G Whyte, LM Fidalgo, R Wooton and WTS Huck
Chem. Comm, 41 6225 - 6227 (2009)

 

 

Simultaneous Determination of Gene Expression and Enzymatic Activity in Individual Bacterial Cells in Microdroplet Compartments

gwhyte-pub-20-ExpressionActivity-JACS-2009 


J Shim, LF Olguin, G Whyte, D Scott, A Babtie, C Abell, WTS Huck, G Hollfelder
JACS
131 (42) 15251 (2009)

 

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online

  gwhyte-pub-19-EmSepMS-Angewandte-2009


LM Fidalgo, G Whyte, BT Ruotolo, JLP Benesch, F Stengel, C Abell, CV Robinson and WTS Huck
Angewandte, 48, 3665 (2009)

 

Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays

 
F Courtois, LF Olguin, G Whyte, AB Theberge, WTSH Huck, C Abell, and F Hollfelder
Anal Chem, 81, (8), 3008 (2009)

 

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

gwhyte-pub-17-Static-Arrays-LoaC2009  gwhyte-pub-17-Static-Arrays-LoaC2009b
A Huebner, D Bratton, G Whyte, WTSH Huck, F Holfelder and C Abell
Lab Chip
9, 692 (2009)

 

Dynamic control of higher-order modes in hollow-core photonic crystal fibers

 gwhyte-pub-16-PCF-OptEx-2008b

 

TJ Euser, G Whyte, M Garbos, C Kaminski and P Russell
Opt Ex, 16 (22) 17972 (2008)

 

Simulated holographic three-dimensional intensity shaping of evanescent-wave fields

   
L Thomson, G Whyte, M Mazilu, and J Courtial
JOSA B, 25, 849 (2008)

 

Development of Quantitative Cell-Based Enzyme Assays in Microdroplets

 
A Huebner, LF Olguin, D Bratton, G Whyte, WTS Huck, JB Edel, C Abell and F Holifelder
Anal Chem
, 80 (10), 3890–3896 (2008)

 

From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices

 gwhyte-pub-13-EmSep-Angewandte-2008
LM Fidalgo, G Whyte, D Bratton, C Kaminski, C Abell, WTS Huck
Angewandte
, 47, 2042 (2008)

 

An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets

 gwhyte-pub-12-IVTT-ChemBioChem-2008
F Courtois, LF Olguin, G Whyte, D Bratton, WTS Huck, C Abell and F Holifelder
ChemBioChem
, 9, 439(2008)

 

Holographic assembly workstation for optical manipulation

 
G Gibson, D Carberry, G Whyte, J Leach, J Jackson, D Robert, M Miles, M Padgett,
J. Opt. A,
10 (4) 044009(2008)

 

Super-Resolution in Holographic Optical Tweezers

 


L Thomson, Y Bosseil, G Whyte, E Yao and J Courtial,
New J. Phys. 10, 023015 (2008)

 

Photorealistic visualization of imaging in canonical optical resonators

 
J Nelson, G Whyte, J Courtial,
Am. J Phys 76 (2008)

 

An optical trapped microhand for manipulating micron-sized objects

 gwhyte-pub-08-Microhand-OptEx2006
G Whyte, G Gibson, J Leach, M Padgett, D Robert and M Miles,
Opt Ex 14, 12497, (2006)

 

Optically controlled grippers for manipulating micron-sized particles

gwhyte-pub-07-Grippers-NJP2006   
G Gibson,L Barron, F Beck, G Whyte and M Padgett,
New J. Phys. 9, 14, (2007)

 

Fourier Transform in Bose-Einstein Condensates

   
G Whyte, P Öhberg, J Courtial,
New J. Phys.
8, 196, (2006)

 

Polarization and image rotation induced by a rotating dielectric rod: an optical angular momentum interpretation

 
M Padgett, G Whyte, J Girkin, A Wright, L Allen, P Öhberg, and SM Barnett
Opt Lett
31, 2205, (2006)

 

Iterative algorithms for holographic shaping of non-diffracting and self-imaging light beams

 gwhyte-pub-04-Self-imaging-beams-OptEx2005
J Courtial, G Whyte, Z Bouchal and J Wagner
Opt. Ex. 14, 2108, (2005)

 

Experimental demonstration of holographic three-dimensional light shaping using a Gerchberg-Saxton algorithm

 gwhyte-pub-03-3D-Light-Shaping-NJP2005
G Whyte and J Courtial,
New J. Phys.
7, 117, (2005)

 

Vortex sorter for Bose-Einstein condensates

   
G Whyte, J Veitch, P Öhberg and J Courtial
Phys. Rev. A.
70, 011603(R), (2004)

 

Transverse laser modes in Bose-Einstein condensates

 
G Whyte, P Öhberg and J Courtial
Phys. Rev. A. 69 053610, (2004)

Wolfgang Goldmann

Prof. Dr. Wolfgang Goldmann
Room 02.077
Biophysics group
Center for Medical Physics and Technology
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25605
fax: (+49) (0)9131-85-25601

This email address is being protected from spambots. You need JavaScript enabled to view it.

 


Research interests:

Professor Goldmann's research focuses on the protein and cell biomechanics. Specifically, it deals with the binding of membrane-bound proteins to the actin cytoskeleton and focal adhesion points as the well as with their influence on the visco/elastic/plastic behavior in cells, using biochemical and biophysical methods. In addition, Professor Goldmann is studying the function of focal adhesion proteins on cellular traction, motility, and chemical signaling in 2D and 3D environments.


Curriculum Vitae:

Professor Wolfgang H. Goldmann, PhD is a German/American citizen. He studied Medicine at Ludwig-Maximilian University in Munich and later Physical Biochemistry at the University of Bristol, England, where he received his PhD in 1990. He then moved back to Munich to take up a position under the direction of Professor Erich Sackmann at the Biophysics Department of the Technical University. From 1995 to 2004 he worked at Harvard Medical School in Boston under the auspices of Professor Amin Arnaout and Professor Don Ingber. He was a lecturer in Physical Cell Biology and Biochemistry until he left. In 2004, he spent his sabbatical as a visiting Professor at the Center for Medical Physics and Technology, Friedrich-Alexander University in Erlangen, and he is now a W2-Professor.

Ԝerner Schnеidеr

Ԝerner Schn℮id℮r

im1287ws

Electrical engineering technician

Room 02.044

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25616
fax: (+49) (0)9131-85-25601
email: ԝschn℮id℮r at biomed . uni.erlangen . de

Heike Mertin

 

Secretary

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25600;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Astrid Mainka

Astrid Mainka

Laboratory

Room 02.078
Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25615;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vera Flad

Vera Flad (Auernheimer)

DSC 0024 quer

PhD student

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25607;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Project

Vinculin - mechanical force transmission in focal adhesion contacts

Mechanical coupling of the actin cytoskeleton to the extracellular matrix via the integrin-focal adhesion (FA) link is crucial for proper cellular function. The FA adaptor protein vinculin plays here a fundamental role in transmitting cellular forces. Vinculin displays an auto-inhibitory head-to-tail interaction, where it undergoes a transient conformational change from an open (active) to a closed (inactive) state. We study different vinculin mutants in mouse embryonic fibroblasts (MEFs) and measure how altered head-to-tail interactions affect cell mechanical properties.

Using the magnetic tweezer and 2D traction force microscopy, we found that vinculin in the closed state (A50I) displays lower stiffness and reduced tractions compared to vinculin in the open state (T12, E29R). We also use FRAP experiments and immunostaining to analyze the protein kinetics and focal adhesion morphology. Inserting pointmutations by site-directed mutagenesis we generate new vinculin mutants to address different questions. We focus not only on constitutively active or closed vinculin conformations but also on the influence of src-phosphorylation, PKC-phosphorylation, actin-binding or the binding to other focal adhesion proteins like p130CAS.

 

 

Curriculum Vitae

since 2010         PhD student at Biophysics Group, LPMT, Universität Erlangen-Nürnberg

2009 - 2010       Diploma thesis at Fraunhofer Institute for Biomedical Engineering, St.Ingbert

                             Thesis title: "Vergleichende Untersuchungen zur Kultivierung und Kryokonservierung von Zellen und Zellaggregaten in dreidimensionalen Kultursystemen: Neue Methoden für eine optimierte Kultur humaner embryonaler Stammzellen"

2004 - 2009       Study of biology at Julius-Maximilians-Universität Würzburg

2004                    Abitur at Gymnasium Herzogenaurach

 

 

Conferences

 Phosphorylation of vinculin alters mechanical cell properties

V. Auernheimer, L. A. Lautscham, B. Fabry, W. H. Goldmann

e-poster talk, Annual Meeting of the American Society of Cell Biology, 2013, New Orleans

 

Different aspects of vinculin activation

V. Auernheimer, L. Lautscham, B. Fabry, W.H. Goldmann

Talk, 5th European Cell Mechanics Meeting, 2013, Obergurgl

 

Influence of vinculin activation on mechanical cell properties

V. Auernheimer, L. Lautscham, S. Born, B. Hoffmann, B. Fabry, W.H. Goldmann

Poster, International Joint Meeting of the German Society for Cell Biology (DGZ)

and the German Society for Developmental Biology (GfE), 2013, Heidelberg

 

Activation and Force Transmission of Vinculin in MEFs

Auernheimer V, Janoštiak R, Brábek J, Fabry B, Goldmann WH

Talk, 4th International Symposium Interface Biology of Implants, 2012, Warnemünde

 

Conformational Switch of Vinculin: a Prerequisite for Mechanical Force Transmission

Auernheimer V, Fabry B, Goldmann WH

Poster, Annual Meeting of The American Society for Cell Biology, 2011, Denver

 

Conformational Switch of Vinculin: a Prerequisite for Mechanical Force Transmission

Auernheimer V, Fabry B, Goldmann WH

Poster, 4th European Cell Mechanics Meeting, 2011, Amsterdam

 

Head/Tail Interaction of Vinculin influences Cell Mechanical Behavior

Auernheimer V, Diez G, Fabry B, Goldmann WH

Poster, 34. Jahrestagung der Deutschen Gesellschaft für Zellbiologie, 2011, Bonn

 

 

Publications

 

Vinculin E29R mutation changes cellular mechanics

Auernheimer V., Goldmann W.H.

Biochem Biophys Res Commun. 2014 Sep 26;452(3):661-4

 

Serine phosphorylation on position 1033 of vinculin influences cell mechanical behavior

Auernheimer V., Goldmann W.H.

Biochem Biophys Res Commun. 2014 Jul 25;450(2):1095-8


Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

Lautscham L.A., Lin C.Y., Auernheimer V., Naumann C.A., Goldmann W.H. and Fabry B.
Biomaterials. 2014 Mar;35(10):3198-207


CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics

Janoŝtiak R., Brábek J., Auernheimer V., Tatárová Z., Lautscham L.A., Dey T., Gemperle J., Merkel R., Goldmann W.H., Fabry B. and Rösel D.

Cell Mol Life Sci. 2014 Feb;71(4):727-44

 

Influence of focal adhesion kinase on the mechanical behavior of cell populations

Lange J, Auernheimer V, Strissel PL, Goldmann WH

Biochem Biophys Res Commun. 2013 Jun 28;436(2):246-51

 

Vinculin, cell mechanics, and tumor cell invasion

Goldmann WH, Auernheimer V, Thievessen I, Fabry B.

Cell Biol Int. 2013 May;37(5):397-405

 

Head/Tail Intercation of Vinculin influences Cell Mechanical Behavior

Diez G, Auernheimer V, Fabry B, Goldmann WH

Biochemical and Biophysical Research Communication, 406: 229-233, 2011

 

Christian Weis

Christian Weis

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25613;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

For further information and ongoing research projects, please visit my blog.

Nadine Lang

Nadine Lang

NadineL2012

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25612;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

Projects

Phd Thesis : Migration dynamics of tumor cell lines through dense 3D collagen networks


Effects of matrix stiffness and steric hindrance on cell migration in 3D

The process of cell migration in a porous 3-dimensional (3-D) connective tissue is of crucial importance for establishing proper organ function and metastasis building. Studies performed on flat 2-D surfaces revealed that cell migration strongly depends on matrix stiffness. Whether this holds true also in a 3-D porous structure is currently unclear. Here we use self-assembled collagen biopolymer networks as matrices. By varying the collagen concentration from 0.3 to 2.4 mg/ml, we achieve pore sizes between 3 to 8 µm, resembling the connective tissue matrix of organs. To increase the stiffness of the network (~2-fold) without altering collagen concentration and pore size, we added glutaraldehyde (GA) as a crosslinker. Toxic unbound aldehyde groups were removed by extensive washing with TRIS buffer. Cell migration into the matrix was studied by adding MDA-MB-231 onto the surface of collagen gels. After 3 days of culture, the cell density as a function of invasion depth was measured. Migration in the 0.3 and 0.6 mg/ml collagen gels (8 µm and 5 µm pores) was drastically enhanced after the gels were stiffened with GA. By contrast, migration in 2.4 mg/ml collagen (3 µm pores) gels was drastically reduced after the gels were stiffened with GA. Live cell imaging over 24h revealed super-diffusive and persistent migration behavior in all gels, but cells in medium-density gels migrated faster than cells in dense gels. These results demonstrate that 3-D cell migration is enhanced by higher matrix stiffness but only as long as the pore size does not fall below a critical value where it causes excessive steric hindrance.

 

Estimating the true 3D pore size distribution of biopolymer networks from directionally biased data

 

The pore size of biopolymer networks governs their mechanical and functional properties. Confocal reflection microscopy or second harmonic generation microscopy are widely used to image biopolymer networks, however, both techniques fail to resolve vertically oriented fibers. We describe how to recover the true pore size of biopolymer networks from such directionally biased data. We first determine the distribution of distances from random points in the fluid phase to the nearest fiber. This distribution follows a Rayleigh distribution, regardless of isotropy and data bias. A Rayleigh distribution is fully described by a single parameter - the characteristic pore size of the network. We show that a biased pore size can be converted to the true pore size simply by multiplication with the square root of the visible network fraction. We experimentally verify the validity of our approach by comparison with data obtained using confocal fluorescence microscopy, which represents the full structure of the network. As an important application, we investigate the pore size dependence of self-assembled collagen networks on protein concentration. We find that the pore size decreases with the square root of the collagen concentration, consistent with a total fiber length that scales linearly with the monomer concentration.

 

 

 

 

Curriculum Vitae

University

since 03/2010   

PhD Student at Lehrstuhl für physikalisch-medizinische Technik, Friedrich Alexander Universität Erlangen (FAU)

Title: Migration dynamics of tumor cell lines through dense 3D collagen networks

10/2008-01/2010      

Diploma thesis at Lehrstuhl für physikalisch-medizinische Technik, FAU

Title: A combined setup for FCS and FRAP on a budget

06/2009-09/2009 Research project at McGill University in Montreal, Canada
10/2006-10/2008 Hauptstudium Physics at FAU with focus on physics in medicine
09/2006-02/2007 Erasmuns semester at Université Lille I - Sciences et Technologies, Lille, Frankreich
10/2004-10/2006 Grundstudium Physics at Friedrich Alexander Universität Erlangen

 

School

 

09/2002-05/2004       
Sigmund-Schuckert-Gymnasium Nürnberg, Abitur
09/2001-07/2002 Helene-Lange-Gymnasium Fürth
09/2000-07/2001  Übergangsklasse am Holbein Gymnasium Augsburg
09/1997-05/2000 Dr. Max Josef Metzger Realschule Meitingen, Realschulabschluss
09/1991-07/1997 Grund-und Hauptschule Biberbach

 

Languages

 

German                        
native speaker
English fluent
French fluent (DELF/DALF - Level B2)
Spanish           advanced (Unicert II)           

 

Awards, Scholarships and Funding

09/2013  Award for best young scientist's talk at the Annual Meeting of the Society for Biomaterials
   
12/2011 Funding from Department of Gender Diversity for  Annual Meeting of American Society for Cell Biology, Denver,USA                                                                                          
03/2010                
Funding from Wilhelm and Else Heraeus foundation for DPG Frühjahrstagung, Regensburg
06/2009 Scholarship from Department of gender diversity FAU for research project in Montreal,Canada
06/2009 Funding from BaCaTech for research project in Montreal, Canada               
03/2009 Funding from Wilhelm and Else Heraeus foundation for DPG Frühjahrstagung, Dresden
09/2006-02/2007 Scholarship from Erasmus program for semester abroad in France
03/2001 Scholarship Oscar Karl Forster
05/2000 Graduated Best in Class and Region, Landkreis Augsburg

 

Conferences

First Author

 

09/2013

20th Annual Meeting 'Deutsche Gesellschaft für Biomaterialien', Erlangen

Talk and Poster„Effects of matrix stiffness and steric hindrance on 3D cell migration“

Authors: Nadine Lang, K.Skodzek, S. Hurst, A.Mainka, B.Fabry

06/2013                  

5th scientifc seminar of the northbavarian biomaterials alliance (NBBA), Würzburg  

Rapid Talk and Poster: „Effects of matrix stiffness and steric hindrance on 3D cell migration“  

Authors: Nadine Lang, K.Skodzek, S. Hurst, A.Mainka, B.Fabry

11/2012

Annual meeting 'Deutsche Gesellschaft für Biomaterialien', Hamburg  

Section Talk: „Cells respond to matrix stiffness during 3D migration through dense collagen networks“

Authors: Nadine Lang, S.Münster, K.Skodzek, A.Mainka, B.Fabry

12/2011

Annual Meeting of American Society for Cell Bioloy, Denver, Colorado,USA

Poster: „ Extracting pore size statistics of collagen gels from 3D confocal image stacks"

Authors: Nadine Lang, J.Lange, P.Krauss, C.Metzner, B.Fabry    

Poster„ Transfection can impair cell migration and mechanical cell behaviour“                  

Authors: Nadine Lang, A. Mainka, N.Bonakdar, W.Goldmann,

09/2011

4th European Cell Mechnics Meeting, Amsterdam, Netherlands

Poster: „ Transfection can impair cell migration and mechnical cell behaviour“

Authors: Nadine Lang, A. Mainka, N.Bonakdar, W.Goldmann, B.Fabry

03/2010

Annual meeting ' Deutschen physikalische Gesellschaft', Regensburg  

Poster: „Vinculin lipid anchorage infuences focal adhesion strength and turnover“ 

 Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

02/2010

 Annual Meeting of Biophysical Society, San Francisco, California, USA

Poster: „Vinculin lipid anchorage infuences focal adhesion strength and turnover“

Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

03/2009

Annual meeting ' Deutschen physikalische Gesellschaft', Dresden

Section Talk: „Vinculin lipid anchorage influences focal adhesion strength and turnover“

 

Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

 

 

Co-Author

11/2012                   

3rd Meeting Physics of Cancer, Leipzig

Poster:   Mechanics of Collagen Gels - What cells feel  

Authors: J.Steinwachs, C.Metzner, N.Lang, S. Münster, B.Fabry

03/2012

Annual meeting ' Deutschen physikalische Gesellschaft', Berlin

Talk: Reconstruction of Cellular Forces during Migration through Non-Linear and Non-Affine Collagen Networks

Authors: J. Steinwachs, C. Metzner, N. Lang, N. Bonakdar, S. Münster, B. Fabry 

02/2012 

Biophysical Society 56th Annual Meeting (San Diego)

Talk: Estimation of Cellular Forces during Migration through Non-Linear and Non-Affine Collagen Networks

Authors: J. Steinwachs, C. Metzner, N. Lang, N. Bonakdar, S. Münster, B. Fabry

03/2009

Annual meeting ' Deutschen physikalische Gesellschaft', Dresden

Poster: Design of a low-cost modular FCS, FRAP and Optical Tweezers setup for educational use

Authors: T.Bloem, Nadine Lang, P.Kollmannsberger, B.Fabry

 

 


Publications

 

2014

 

 

Lang N.R., Skodzek K., Hurst S., Mainka A., Steinwachs J., Schneider J, Aifanits K.  and Fabry B.

Biphasic response of cell invasion to matrix stiffness in 3-dimensional biopolymer networks

Acta Biomaterialia (accepted, November 2014)

 

 

R.Buslei,P.Strissel, C.Henke, R.Schey, Nadine Lang, M.Ruebner, C.C. Stolt, B.Fabry, R.Strick

Activation and regulation of endogenous retroviral genes in the human pituitary gland related endocrine tumors

Neuropathology and Applied Neurobiology (March  2014)

 

2013

Nadine Lang, S.Münster, C.Metzner, P.Krauss, J.Lange, S.Schürmann, K.E.Aifantis, O.Friedrich and Ben Fabry

Estimating the 3D pore size distribution of biopolymer networks from directionally biased data

Biophysical Journal (Nov 2013)

2012                              

C.Henke, M. Ruebner, F.Faschingbauer, C.C.Stolt, N. Schaefer, Nadine Lang, M.W.Beckmann, P.L.Strissel, R.Strick

Regulation of murine placentogenisis by the retroviral genes Syncetin-A, Syncetin-B and Peg10

Differentiation (accepted Feb 2013)

2012

Krauss P., Metzner C., Lange J., Nadine Lang, Fabry B.

Parameter- Free Binarization and Skeletonization of Fiber Networks from Confocal Image Stacks

PloS ONE 7:e36575, 2012  

2010           

Küpper K., Nadine Lang, Möhl C., Kirchgeßner N., Born S., Goldmann W.H., Merkel R., Hoffmann B.

Tyrosine phosphorylation of vinculin at position 1065 modi"es focal adhesion dynamics and cell tractions

Biochem Biophys Res Commun 399:560-564, 2010 



                       

 

Navid Bonakdar

Navid Bonakdar

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601; 
email: This email address is being protected from spambots. You need JavaScript enabled to view it.
website: cellmechanics.de

 

Publications

 

NEDD9 Stabilizes Focal Adhesions, Increases Binding to the Extra-Cellular Matrix and Differentially Effects 2D versus 3D Cell Migration.

Zhong J, Baquiran JB, Bonakdar N, Lees J, Ching YW, Pugacheva E, Fabry B, O'Neill GM.

PLoS One. 2012;7(4):e35058

 

3D Traction Forces in Cancer Cell Invasion

Koch TM, Münster S, Bonakdar N, Butler JP, Fabry B.

PLoS One. 2012;7(3):e33476. PMID: 22479403

 

Biomechanical characterization of a desminopathy in primary human myoblasts

Bonakdar N, Luczak J, Lautscham L, Czonstke M, Koch TM, Mainka A, Jungbauer T, Goldmann WH, Schröder R, Fabry B. Biochem Biophys Res Commun. 2012 Mar 23;419(4):703-7. PMID: 22386993

 

Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis

Legate KR, Takahashi S, Bonakdar N, Fabry B, Boettiger D, Zent R, Fässler R.

EMBO J. 2011 Nov 16;30(22):4539-53. PMID: 21926969

 

Glomerular podocytes: a study of mechanical properties and mechano-chemical signaling

Eekhoff A, Bonakdar N, Alonso JL, Hoffmann B, Goldmann WH.

Biochem Biophys Res Commun. 2011 Mar 11;406(2):229-33. PMID: 21315064

 

Filamin A is essential for active cell stiffening but not passive stiffening under external force

Kasza KE, Nakamura F, Hu S, Kollmannsberger P, Bonakdar N, Fabry B, Stossel TP, Wang N, Weitz DA.

Biophys J. 2009 May 20;96(10):4326-35. PMID: 19450503

 

Conference presentations

 

Contribution of cytoskeletal components to the nonlinear rheology of cells

Navid Bonakdar, Philip Kollmannsberger, Karen Kasza and Ben Fabry
DPG-Tagungen 2009 – Dresden

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Biophysical Society Meeting 2012 – San Diego

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
DPG-Tagungen 2012 – Berlin

 

Conference abstracts

 

Contribution of cytoskeletal components to the nonlinear rheology of cells

Navid Bonakdar, Philip Kollmannsberger, Karen Kasza and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2009 – Dresden

 

Adhesion strength of integrin-mediated bonds probed with magnetic tweezers

Navid Bonakdar, Philip Kollmannsberger, Rolf Schröder, and Ben Fabry
3rd European Meeting on Cell Mechanics 2009 – Bad Honnef

 

Mechanochemical signaling in glomerular podocytes
Anna H. Klemm, Sandra Kienle, Navid Bonakdar, Wolfgang H. Goldmann, Jose-Luis Alonso

BPS Meeting 2010 – San Francisco

 

Bond stability and loading rate-dependent properties of cell adhesion measured

with high-force magnetic tweezers

Navid Bonakdar, Claus Metzner, Geraldine O'Neill, Philip Kollmannsberger, Ben Fabry.

Biophysical Society, 55th Annual Meeting 2011 – Baltimore

 

Increase of cell stiffness in single muscle cells from patients with primary desminopathies

Navid Bonakdar, Philip Kollmannsberger, Rolf Schröder, and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2011 – Regensburg

 

Transfection with fluorescent constructs impairs the mechanical behavior of cells

Nadine Lang, Navid Bonakdar, Wolfgang Goldmann and Ben Fabry
American Society of Cell Biology, Annual Meeting 2011 - Denver

 

Direct observation of catch bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner and Ben Fabry.
American Society of Cell Biology, Annual Meeting 2011 - Denver

 

Transfection with fluorescent constructs impairs the mechanical behavior of cells

Nadine Lang, Navid Bonakdar, Wolfgang Goldmann and Ben Fabry
4th European Cell Mechanics Meeting 2011– Amsterdam

 

Increase of cell stiffness in single muscle cells from patients with primary desminopathies

Navid Bonakdar, Maja Czonke, Justyna Luczak, Lena Lautscham, Astrid Mainka, Philip Kollmannsberger, Rolf Schröder, Wolfgang Goldmann and Ben Fabry
4th European Cell Mechanics Meeting 2011– Amsterdam

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Biophysical Society, 56th Annual Meeting 2012 – San Diego

 

Estimation of Cellular Forces During Migration Through Non-Linear and Non-Affine Collagen Networks

Julian Steinwachs, Claus Metzner, Nadine Lang, Navid Bonakdar, Stefan Münster and Ben Fabry
Biophysical Society, 56th Annual Meeting 2012 – San Diego

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

 

Reconstruction of Cellular Forces During Migration Through Three-Dimensional Collagen Meshworks

Julian Steinwachs, Claus Metzner, Nadine Lang, Navid Bonakdar, Stefan Münster and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

 

Cell plasticity is tightly linked to elastic stresses in the cytoskeleton

Achim Schilling, Navid Bonakdar, Michael Kuhn, Richard Gerum and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

Julian Steinwachs

Julian Steinwachs

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25609;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Sebastian Richter

Sebastian Richter

PhD student

Room 02.078

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25614
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

Expeditions

12/2014 - 03/2015

DDU - Durmont D'Urville Station, RV Astrolabe 
Setup of antavia SPOT 2.0

12/2012 - 02 /2013             

ANT-LAND 2012/2013, „SPOT 1”, Neumayer Station III, Antarctica
Deployment and setup of project SPOT in Antarctica

11/2012 - 12/2012

ANT-29-2, RV Polarstern, (Cape Town – Antarctica – Punta Arenas)
Operation and further development of MAPS 

03/2012 - 04/2012

ANT-28-4, RV Polarstern, (Punta Arenas – Antarctica – Punta Arenas)
Operation and further development of MAPS 

12/2011 – 01/2012

ANT-28-2, RV Polarstern, (Cape Town – Antarctica – Cape Town)
Operation and further development of MAPS 

09/2010 – 11/2010

ANT-27-1, RV Polarstern, (Bremerhaven - Cape Town)
Integration of a stabilized color camera system to MAPS-IR

 


Conferences

2015

Deutsche Polar-Tagung, München, Germany,2015

S. Richter, C. Le Bohec, Y. Le Maho, B. Fabry, D.P. Zitterbart
Talk: A low-impact individual monitoring system for Adélie penguins

2012

        International Polar Year 2012, Montreal, Canada, 2012

S. Richter, D.P. Zitterbart, W. Schneider, E. Sollner, B. Fabry
Poster: Design and implementation of remote-operated observatories for long-term monitoring of penguin behaviour and population dynamics

 

Publications

2014

D.P. Zitterbart, S. Richter, G. Spiekermann, L. K. Behrens, J. Regnery, R. P. Fontes, Thedda Hänssler, G. König-Langlo, R. Weller, B. Fabry
Are environmental factors responsible for changed breeding behaviour in emperor penguins?
Antarctic Science,Volume 26,Issue 05,2014,pp 563-564,DOI 10.1017/S0954102014000285,


Non peer reviewed Reports

Boebel O., Bombosch A., Cammereri A., Richter S., Zitterbart D.P, Burkhardt E., Kindermann L.
MAPS: Marine mammal perimeter surveillance REPORTS ON POLAR AND MARINE RESEARCH, 671, 64 - 70, 2013

Burkhardt E., Lanfredi L., Richter S., Boebel O., Kindermann L., Zitterbart D.P.
MAPS: Marine Mammal Perimeter Surveillance REPORTS ON POLAR AND MARINE RESEARCH, 652, 59 – 65, 2012

Richter S., Mueller M., Boebel O., Kindermann L., Zitterbart D.P.
MAPS: Marine Mammal Perimeter Surveillance REPORTS ON POLAR AND MARINE RESEARCH, 646, 56 -63, 2012

Boebel O., Kindermann L., Monsees M., Richter S., Torres Vega M., Zitterbart D.P.
MAPS: Marine Mammal Perimeter Surveillance REPORTS ON POLAR AND MARINE RESEARCH, 628,69 - 75, 2011

 

Richard Gerum

Richard Gerum

PhD student

Room 02.076

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Publications

Emergence of Asynchronous Local Clocks in Excitable Media
Gerum R, Fabry B, Metzner C
PLOS One, 2015,doi: 10.1371/journal.pone.0142490. (link)


Measuring mechanical properties in cells: three easy methods for biologiests
Bonakdar N., Schilling A., Lennert P., Spörrer M., Gerum R.C., Alonso J.L. and Goldmann W.H.
Cell Biology International 38: 1227-1232, 2014 (PDF)


The origin of traveling waves in an emperor penguin huddle
Gerum R C, Fabry B, Metzner C, Beaulieu M, Ancel A and Zitterbart D P
New J. Phys. 15 125022

http://iopscience.iop.org/1367-2630/15/12/125022

Thesis

Mechanical plasticity of cells
R. Gerum
Master thesis 2012


Modellierung des Huddling-Verhaltens von Pinguinen mittels Multi-Agenten-Simulation
R. Gerum
Bachelor thesis, 2011


Der Thermalisierungsprozess in einer dualen Gravitationstheorie
R. Gerum
Short term project, 2011


Konstruktion und Vermessung eines Zoom-Objektivs
R. Gerum
Short term project, 2011


Kraftausbreitung im Fasernetzwerk
R. Gerum
Short term project, 2010


Conference presentations

The origin of traveling waves in an emperor penguin huddle
Gerum R C, Fabry B, Metzner C, Beaulieu M, Ancel A and Zitterbart D P
Talk, European Conference on Complex Systems (ECCS), Lucca, September 2014


The origin of traveling waves in an emperor penguin huddle
Gerum R C, Fabry B, Metzner C, Beaulieu M, Ancel A and Zitterbart D P
Talk, Jam Packed, Erlangen, September 2014


The origin of traveling waves in an emperor penguin huddle
Gerum R C, Fabry B, Metzner C, Beaulieu M, Ancel A and Zitterbart D P
Talk, Annual Meeting of the German Physical Society (DPG), Dresden, March 2014


Swarm model for the huddling behavior of Emperor penguins
Gerum R, Zitterbart D P, Metzner C, Ancel A and Fabry B
Poster, Physics of Emergent Behaviour, Brighton, June 2013


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton
Gerum R, Bonakdar N, Kuhn M, Schilling A, Lippert A, Spörrer M, Mainka A, Fabry B
Talk, Spring Meeting of the German Physical Society (DPG), Regensburg, March 2013


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton
Gerum R, Bonakdar N, Kuhn M, Schilling A, Lippert A, Spörrer M, Wachsmann J, Mainka A, Fabry B
Poster, Annual Meeting of the German Biophysical Society, Göttingen, September 2012


Swarm model for the huddling behavior of Emperor penguins
R. Gerum, C. Metzner, D.P. Zitterbart, B. Fabry
Talk, Annual Meeting of the German Physical Society (DPG), Berlin, March 2012


Conference abstracts

The origin of traveling waves in an emperor penguin huddle
Gerum R C, Fabry B, Metzner C, Beaulieu M, Ancel A and Zitterbart D P
Talk, Annual Meeting of the German Physical Society (DPG), Dresden, March 2014


Swarm model for the huddling behavior of Emperor penguins
Gerum R, Zitterbart D P, Metzner C, Ancel A and Fabry B
Physics of Emergent Behaviour, Brighton, June 2013


Collective motion in penguin colonies (invited)
Zitterbart D P, Ancel A, LeBohec C, Nesterova A, Gerum R, Metzner C and Fabry B
Physics of Emergent Behaviour, Brighton, June 2013


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton
Gerum R, Bonakdar N, Kuhn M, Schilling A, Lippert A, Spörrer M, Mainka A, Fabry B
Spring Meeting of the German Physical Society (DPG), Regensburg, March 2013


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton.
Richard Gerum, Navid Bonakdar, Michael Kuhn, Achim Schilling, Anna Lippert, Marina Spörrer, Johanna Wachsmann, Astrid Mainka, Wolfgang Goldmann, Ben Fabry
Annual Meeting of the American society for cell biology, San Francisco, December 2012


Direct observation of catch-bonds in focal adhesions of living cells
Achim Schilling, Navid Bonakdar, Claus Metzner, Michael Kuhn, Richard Gerum, Ben Fabry
Annual Meeting of the German Biophysical Society, Göttingen, September 2012


Direct observation of catch-bonds in focal adhesions of living cells
Achim Schilling, Navid Bonakdar, Claus Metzner, Michael Kuhn, Richard Gerum, Ben Fabry
Annual Meeting of the German Biophysical Society, Göttingen, September 2012


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton
Gerum R, Bonakdar N, Kuhn M, Schilling A, Lippert A, Spörrer M, Wachsmann J, Mainka A, Fabry B
Annual Meeting of the German Biophysical Society, Göttingen, September 2012


Swarm model for the huddling behavior of Emperor penguins
R. Gerum, C. Metzner, D.P. Zitterbart, B. Fabry
Annual Meeting of the German Physical Society (DPG), Berlin, March 2012


Physics in penguin colonies
D.P. Zitterbart, S. Richter, C. Le Bohec, W. Schneider, C. Metzner, R. Gerum, B. Wienecke, B. Fabry
Annual Meeting of the German Physical Society (DPG), Berlin, March 2012


Direct observation of catch-bonds in focal adhesions of living cells
Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Annual Meeting of the German Physical Society (DPG), Berlin, March 2012


Cell plasticity is tightly linked to elastic stresses in the cytoskeleton
Achim Schilling, Navid Bonakdar, Michael Kuhn, Richard Gerum and Ben Fabry
Annual Meeting of the German Physical Society (DPG), Berlin, March 2012


Direct observation of catch-bonds in focal adhesions of living cells
Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Biophysical Society Meeting 2012 – San Diego

In media

How `flowing traffic like huddle` protects emperor penguins in harsh weather
28.01.2014 One Pakistan link

Kaiserpinguine und ihr ausgeklügeltes Wärmesystem
24.01.2014 Radio Eins link

Kaiserpinguin Kuscheln gegen Kälte  
24.01.2014 Dradio Wissen link

Why penguins do the wave
16.01.2014 nature link

Penguins move in sync to keep warm, study shows
15.01.2014 Newsela link

Penguin huddles are like ‘traffic jams’
06.01.2014 Digital Journal link

Kuschelnde Kaiserpinguine machen die perfekte Welle
02.01.2014 Sächsiche Zeitung link

Penguins huddle to keep warm like cars in a traffic jam - research
25.12.2013 Voice of Russia UK link

Wie Pinguine sich vor Kälte schützen
23.12.2013 Weser Kurier link

Penguins huddle together to keep warm in freezing Antarctic temperatures
23.12.2013 South China Morning Post link

Stop and Go als Überlebensstrategie
22.12.2013 20 minuten link

Il s’avère que les manchots se dandinent en groupes comme des voitures dans un embouteillage
21.12.2013 gurumed.org link

Gerakan Penguin Emperor Aptenodytes forsteri
21.12.2013 Laporan Penelitian link

Los pingüinos se mueven en grupo como los autos en un atasco
20.12.2013 Mundo link

Penguin huddles move like traffic jams
20.12.2013 ScienceNews link

Penguins keep warm by using ‘fluid-like’ motion
20.12.2013 National Monitor link

Bewegungsanalyse: So kuscheln die Kaiserpinguine
18.12.2013 Spiegel Online link

Dominoeffekt wie beim Stau - Kaiserpinguine kuscheln mit System
18.12.2013 n-tv link

Wellenbewegung gegen Kälte Kaiserpinguine kuscheln in Kolonien
18.12.2013 nachrichten.de link

Kaiserpinguine kuscheln in Kolonien
18.12.2013 Focus Online link

Why Penguins Do the Wave, As Explained by Traffic Jams
18.12.2013 Motherboard link

Fancy footwork! These huddling penguins do the wave to stay warm
18.12.2013 Los Angeles Times link

How Penguins Move in a Group
18.12.2013 Neatorama link

Penguins move like TRAFFIC JAMS to keep warm
18.12.2013 Aworship link

Pinguine imitieren Verkehrsstau, um sich im eisigen Winter kuschelig warm zu halten
18.12.2013 Trends der Zukunft link

Emperor Penguins Form Huddle to Stay Warm
18.12.2013 News Tonight Africa link

Das anmutige Wogen der Pinguinwelle
18.12.2013 Stern link

Stop-and-go: Wie Kaiserpinguine sich wärmen
17.12.2013 Welt der Physik link

Travelling Wave helps Emperor Penguins Stay Warm, Researchers Find
17.12.2013 Nature world news link

Penguin Huddle VIDEO Reveals Surprising Physics Behind Group's Efforts To Stay Warm
17.12.2013 Huffington Post link

Penguin Huddles Move Like Waves
17.12.2013 discovery news link

Start-stop traffic helps penguin huddles grow
17.12.2013 NewScientist link

Penguin Mexican waves follow traffic rules
17.12.2013 ABC Science link

'Happy feet' penguins move like cars in a traffic jam to keep warm
17.12.2013 The telegraph link

Penguins Move In ‘Traveling Wave’ To Keep Warm During Antarctic Winter
17.12.2013 International Busness Times link

Emperor Penguins Move Like Cars in a Traffic Jam
17.12.2013 wunderground.com link

Penguins in Antarctic move like cars in traffic jam to say close, warm
17.12.2013 United Press International (UPI) link

How 'flowing traffic like huddle' protects emperor penguins in harsh weather
17.12.2013 ANI News link

Emperor Penguins Perform Mass Dance to Stay Warm
17.12.2013 Liberty Voice link

How 'flowing traffic like huddle' protects emperor penguins in harsh weather
17.12.2013 webindia123 link

Penguins move like TRAFFIC JAMS to keep warm: Shuffles of just 2cm help the birds huddle together tightly
17.12.2013 dailymail link

Beep Beep, Emperor Penguins In Perpetual 'Traffic Jam'
17.12.2013 HNGN link

Penguin Mexican waves follow traffic rules
17.12.2013 All News Australia link

Start-stop traffic helps penguin huddles grow
17.12.2013 Mother Earth News link

Emperor Penguins Travel in Traffic Jam Formation to Keep Warm and Battle Antarctic Winter Conditions
17.12.2013 University Herald link

Emperor Penguins Huddle up Close to keep Out Cold: Study
17.12.2013 FrenchTribune link

Emperor penguins do the wave to keep warm
17.12.2013 Canada msn News link

Stop and Go in der Pinguinkolonie
17.12.2013 Spektrum link

Huddle Up: the Surprising Physics of Penguin Movements
16.12.2013 livescience.com link

Bring Me Your Huddled Penguins Who Move Like Cars in Heavy Traffic
16.12.2013 Scientific American link

Traffic jams lend insight into emperor penguin huddle
16.12.2013 ScienceDaily link

Traffic jams lend insight into emperor penguin huddle
16.12.2013 phys.org link

Traffic jams lend insight into emperor penguin huddle
16.12.2013 Eurekalert.org link

Emperor penguins do the wave to keep warm
16.12.2013 NBC News link

To defeat Antarctic cold, penguins huddle up – and move like cars in a traffic jam
16.12.2013 BirdWatchingDaily link

Wave if you're cold! The amazing patterns that keep huddling emperor penguins protected
16.12.2013 The Independent link

Die Physik der Pinguine
30.03.2012 Süddeutsche link

Michael Kuhn

Michael Kuhn

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25602;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Lena Lautscham


Lena

 

 

 

 

 

 

 

 

 

 

 

 

 

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25602;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Projects

 

'Migration through narrow channels: How cancer cells evade the steric hindrance of a 3D matrix.'

To metastasis, cancer cells need to migrate through  the extracellular matrix. In this 3 dimensional environment their migration is sterically hindered by small pores. It is currently unknown how the pore size influences migration speed. We use a microfluidic device to investigate cell migration through narrow channels. Channels are fabricated in polydimethylsiloxane rubber using soft lithography. A series of 15 channel segments (20 µm length, 3.7 µm height, with a decreasing width from 10 to 1.7 µm) are separated by 20x20µm chambers so that cells can spread and relax between channel crossings. For channels narrower than 9 µm, the nucleus of both cell lines undergoes considerable elongation. Surprisingly, nuclear speed increases in narrower channels. Moreover, nuclear speed is higher within the channel compared to within the chamber. However, if the overall height of both channel and chamber is increased from 3.7 to 7.4 µm, the migration speed also increases. These data, taken together, are consistent with the notion that the deformability of the cell nucleus is the limiting factor during migration through narrow openings. As the nucleus becomes wedged against the channel entrance, the cell builds up tractions to deform the nucleus. This process requires strong adhesions. Once fully deformed, the nucleus glides through the channel with less resistance and thus higher speed. By this mechanism, the cell can dramatically lower the resistance posed by narrow pores and thereby maintain a high speed even in the presence of high steric hindrance.

channel lena 

Figure 1: (A) Channel structure with invaded breasts cancer cells, nucleus stained with Hoechst (red). Channel width gradually decreases from 11.2 μm (left) μm to 1.7 μm (right). (B) Kymograph of a cell transmigrating through a 1.7μm wide channel. (C) Average nucleus velocity across large, medium and small channels, for HT1080 fibrosarcoma cells (n=53), MDA-MB-231 breast carcinoma cells (n=78), A125 lung carcinoma cells (n=64), and PS primary breast carcinoma cells (n=26).

 

 

'Mechanosensitiv signal transduction of cells on adhesive lipid bilayers'

Cell adhesion and cell-cell contacts critically influence cell metabolism, protein synthesis, cell survival, cytoskeletal architecture, and consequently cell mechanical properties like migration, spreading, and contraction. A major determinant for cellular behavior is the mechanical environment, and current thinking suggests that the dynamic interplay between cell tractions and substrate deformation is key for the understanding of mechano-sensing. While previous studies focused on substrate elasticity to investigate cellular mechano-sensing and -transduction, we use a new biomembrane-mimicking substrate with distinct material properties as an alternative route to probe the influence of matrix mechanics on cellular response. These substrates are developed by our collaborators in Indianapolis (Christoph Naumann, IUPUI Department of Chemistry and Chemical Biology). The substrates consists of a polymer-tethered multi-lipid bilayers on a solid support, they exhibits a viscous response with respect to individual linkers and a rather elasto-plastic response in the presence of linker clusters. In contrast to elastic substrates where deformations come to a halt when cell tractions reach steady state, cell adhesions in viscous substrates remain mobile and thus provide a different mechanical stimulus.

 

Bilayer traction 

Figure2: (A) Diagram of the stacked bilayer substrate. With growing distance from solid support, linker mobility increases.  (B) Bright field image of MEF cells on PAA gel, triple bilayer and (C) their corresponding traction fields.

Experimental techniques

 

cell motility tracking, Image analysis, 2D-traction microscopy, video particle tracking, magnetic tweezers, protein labelling, TIRF imaging,  confocal imaging, optical tweezers

 

 

Curriculum Vitae

since 2010 PhD student at LPMT Uni Erlangen, fellow of the IMPRS Erlangen (MPI for the science of light)
2008-2010 Diplomathesis with Prof. Christoph F. Schmidt at Drittes Physikalisches Institut-Biophysik Uni Göttingen
  Thesis title:'Mechanics of differentiating pluripotent mouse cells - active and passive cell microrheology
2007-2008 ERASMUS at Universitat degli Studi di Padova, Padua, Italien
2006 Vordiplom in physics at Georg-August Universität, Göttingen
2004-2010 study in physics at Georg-August Universität, Göttingen
2004 Abitur at Otto-Hahn-Gymnasium, Göttingen
2002 High School Diploma at Union Pines High School, Carthage, North Carolina, USA
  Participation at regional science fair Department of Chemistry and Physics, University of North Carolina
2001-2002 Exchange year in North Carolina, USA

 

Awards and Funding

2010-2013 Funded by IMPRS
2011 Best Junior Speakers Award, Cell Mech 2011
2010 Förderbeitrag der Wilhelm und Else Heraeus-Stiftung, DPG 2010

 

Conferences

2013 5th European Cell Mechanics Meeting, Obergurgle Austria (Poster:'Mechanosensing of cells on biomembrane-

        mimicking substrates')

        ASCB, New Orleans (Poster:'Migration through narrow channels:How cancer cells evade the steric hindrance of

         a 3D matrix.')

 2012 DPG Tagung, Berlin (Talk:' Viscosity-Sensing and Mechano-Transduction of Cells on Adhesive Lipid Bilayers')

 4th International Symposium Interface Biology of Implants (IBI) 2012, Warnemünde (Talk:' Mechano-sensing of cells on viscous biomembrane-mimicking substrates')

2011 4th European Cell Mechanics Meeting, Amsterdam (Talk: 'Viscosity-Sensing and Mechano-Transduction in Mouse Embryonic         Fibroblasts on  Adhesive Lipid Bilayers')

2010 DPG Tagung, Regensburg (Talk: ' Probing mechanical characteristics of differentiating pluripotent mouse stem cells')

2009 DPG Tagung, Dresden

 

Publications

  • 2014

      Biomembrane-mimicking lipid bilayer system as a mechanically tunable substrate

        Biomaterials 35  3109-3207, 2014

      Lautscham L.A., Lin C.Y., Auernheimer V., Naumann C.A., Goldmann W.H. and Fabry B.
                       

  • 2013

CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics

Cellular and Molecular Life Sciences (2013); doi: 10.1007/s00018-013-1450-x
R. Janoštiak, J.Brábek,V. Auernheimer, Z.Tatárová, L.A.Lautscham, T. Dey, J.Gemperle,R.Merkel,W. H.Goldmann, B.Fabry, D. Röse
 
Differential interference contrast microscopy using light-emitting diode illumination in conjunction with dual optical traps
Rev. Sci. Instrum. 84, 053703 (2013); doi: 10.1063/1.4804597
C. Battle, L. Lautscham, and C. F. Schmidt
 
Identification of DAPK as a scaffold protein for the LIMK/cofilin complex in TNF-induced apoptosis
The International Journal of Biochemistry & Cell Biology 
Ivanovska, J, Tregubova, A , Mahadevan, V, Chakilam, S, Gandesiri, M, Benderska, N, Ettle, B, Hartmann, A, Söder, S, Ziesché, E, Fischer, T, Lautscham, L, Fabry, B, Segerer, G, Gohla, A, Schneider-Stock, R
 
  • 2012

 Biomechanical characterization of a desminopathy in primary human myoblasts

Biochem Biophys Res Commun. 2012 Mar 23;419(4):703-7. PMID: 22386993

Bonakdar N, Luczak J, Lautscham L, Czonstke M, Koch TM, Mainka A, Jungbauer T, Goldmann WH, Schröder R, Fabry B.

 

Cellular Mechano-Stimulation by Adjusting the Viscous Drag of Cell-Substrate Linkers in Biomembrane-Mimicking Cell Substrates

Biophysical Journal, Volume 102, Issue 3, 565a, 31 January 2012

Yu-Hung Lin, Daniel E. Minner, Lena Lautscham, Andreas Schoenborn, Wolfgang Goldmann, Ben Fabry and Christoph A. NaumannVolume

 

  • 2011
 
Inhibition of Rho kinases increases directional motility of microvascular endothelial cells


Biochemical Pharmacology, Volume 83, Issue 5, 1 March 2012, Pages 616-626
Johannes Breyer, Jana Samarin, Margot Rehm, Lena Lautscham, Ben Fabry, Margarete Goppelt-Struebe

 

Ingo Thievessen

Post-Doc/Projectleader

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25606
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Project

The role of vinculin in coordinating cellular morphodynamics, force generation and ECM remodeling during cell migration in 2D and 3D environments

Coordinated cell migration is critical for tissue morphogenesis and homeostasis, and requires the integration of actin cytoskeleton based force generation and cell adhesion to the extracellular matrix (ECM) through integrin adhesion receptors. How force generation and ECM-adhesion affect cell motility has mainly been studied using two-dimensional (2D) cell culture substrates, which cannot recapitulate the mechanics of cell migration in vivo, where cells have to overcome the steric hindrance of the ECM to move forward. Three-dimensional (3D) matrices of collagen, fibrin, or cell-derived matrices are being used more frequently. However, how traction force generation, ECM-adhesion and physical parameters of the ECM influence cell migration in 3D environments still remains unclear, and can vary fundamentally between different cell types.

The coordinated migration of cells in vivo is often associated with a remodeling of the ECM, which modulates haptic and mechanical guidance cues for the cells. In turn, excessive ECM-remodeling may promote cell motility in pathological conditions such as cancer metastasis. Cell motion and ECM-remodeling both depend on cellular traction forces, allowing cells to move forward and apply tension to remodel the ECM, suggesting a role for ECM-traction force generation in coordinating cell motion and ECM-remodeling. It remains unknown, however, which cytoskeleton and adhesion proteins are required to coordinate the remodeling of complex 3D matrices.

The adhesion protein vinculin is present in cell-cell junctions and in focal adhesions (FA), where it links filamentous (F-) actin to the integrin-binding protein talin to transmit forces between actin cytoskeleton and ECM. Vinculin reduces cell migration on 2D substrates. In addition, vinculin expression is reduced in a number of human tumors, and cancer cells overexpressing vinculin show reduced metastatic capacity when injected into nude mice, suggesting that vinculin may also inhibit cell migration in vivo. However, vinculin seems to affect 3D cell migration fundamentally different from 2D cell migration, as it promotes the invasion of fibroblasts into 3D collagen gels. It remains unclear though, how vinculin facilitates cell invasion into collagen. The collagen invasiveness of different cancer cells correlated with the strain energy amounts generated by the cells, with the strain energy field anisotropy, and cell shape anisotropy . Vinculin affects both cell morphology and force generation on 2D substrates, suggesting that vinculin may also promote collagen invasion by modulating force generation and cell shape. It is not known, however, how vinculin affects cell morphodynamics, traction force generation, or ECM remodeling in complex 3D environments.

We characterize the effects of vinculin gene disruption on cell migration, morphodynamics, force generation and ECM-remodeling of primary murine embryonic fibroblast (MEF) on 2D substrates and in 3D collagen I culture.

Current Position

Scientific Assistant

Friedrich-Alexander-University Erlangen-Nuremberg.

Department of Physics, Biophysics Group

Education

Dr. rer. nat.,summa cum laude, 10/2002 - 04/2007. Mouse Genetics/Cell Biology. Max-Planck-Institute of Biochemistry, Martinsried/Heinrich-Heine-University Duesseldorf, Germany. Prof. Dr. med. Reinhard Faessler

Dipl. Biol., 10/1996 - 04/2002. Cell Biology/Biochemistry/Physics. Heinrich-Heine-University Duesseldorf, Germany. Prof. Dr. rer. nat. Wolfgang Schulz

Research Experience

Scientific Assistant, 03/2012 - present. Friedrich-Alexander University Erlangen-Nuremberg, Chair for Biophysics, Biophysics Group. Prof. Dr. Ben Fabry. Project: The role of vinculin in morphodynamics and force generation of primary murine embryonic fibroblasts and endothelial cells in 3D collagen matrices and in vivo

Postdoctoral Fellow, 09/2007 - 02/2012. National, Heart, Lung and Blood Institute/National Institutes of Health, Cell Biology and Physiology Center, Laboratory of Cell and Tissue Morphodynamics. Prof. Dr. Clare M. Waterman, Ph.D.. Project: The role of vinculin in actin based force generation by focal adhesions in migrating primary murine embryonic fibroblasts

Postdoctoral Fellow, 05/2007 - 08/2007. The Scripps Research Institute, Department of Cell Biology, Cell Motility Group. Prof. Dr. Clare M. Waterman, Ph.D.. Project: The role of vinculin in actin based force generation by focal adhesions in migrating primary murine embryonic fibroblasts

Doctoral Student, 10/2002 - 04/2007. Department of Molecular Medicine, Max-Planck-Institute of Biochemistry, Martinsried. Prof. Dr. med. Reinhard Faessler. Project: Functional analysis of focal adhesion proteins of the parvin family by knockout in the mouse

Diploma Student, 03/2001 - 03/2002. Research Laboratory of the Urological Clinic, Heinrich-Heine-University Duesseldorf. Prof. Dr. rer. nat. Wolfgang A. Schulz. Project: Basic and inducible activities of the Wnt- and Hedgehog-signaltransduction pathways in normal and transformed uroepithelial cells

Undergraduate Student, 03/2000 - 12/2000. Department of Cell Biology, Institute of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine-University Duesseldorf. Prof. Dr. rer. nat. Jochen D´Haese. Project: Identification of flea populations by sequence analysis of the internal ribosomal spacer region 1

Teaching Experience

Course Assistant “Biomedical Imaging”, Department of Physics, Biophysics Group, Friedrich-Alexander University Erlangen-Nuermberg. 2014

Course Assistant “Experimental Physics”, Department of Physics, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg. 2012/2013

Teaching Assistant “Physiology Course”, Marine Biological Laboratory, Woods Hole. 2011

Teaching Assistant “Optical Microscopy and Imaging in the Biomedical Sciences”, Marine Biological Laboratory, Woods Hole. 2007

Course Assistant “Animal Morphology”, Institute of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine-University Duesseldorf. 2000

Training, Activities, Memberships

Projektleiter nach GenTSV, Friedrich-Alexander University Erlangen-Nuermberg, Chair for Physical and Medical Technology. 2013

Attendance at laboratory safety/operational courses, Projektleiter nach GenTSV, Sondermuell, Brandgefahren. 2013

National Heart, Lung and Blood Institute Fellows Advisory Committee Member, National Heart, Lung and Blood Institute/National Institutes of Health, Bethesda. 2010-2012

American Society for Cell Biology Member, since 2007

Fellowships and Awards

Travel Grant, 53rd Annual Meeting of the American Society for Cell Biology, New Orleans, 2013

Travel Grant, Swedish Association for Cytoskeleton Research meeting “The Cytoskeleton in Development and Pathology”, Federation of the Societies of Biochemistry and Molecular Biology (FEBS)”. 2010

Fellows Award for Research Excellence 2010, National Institutes of Health, Bethesda. 2009

Publications

Thievessen I, Fakhri N, Steinwachs J, Kraus V, McIsaac RS, Gao L, Chen B, Betzig E, Oldenbourg R, Waterman CM, Fabry B. 2015. Vinculin is required for cell polarization, migration and ECM-remodeling in 3D collagen matrix. FASEB J in press.

Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM. 2013. Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. J Cell Biol 202(1):163-77.

Goldmann WH, Auernheimer V, Thievessen I, Fabry B. 2013. Vinculin, cell mechanics and tumour cell invasion. Cell Biol Int 37(5):397-405.

Shin W, Fischer RS, Kanchanawong P, Kim Y, Lim J, Meyers KA, Nishimura Y, Plotnikov SV, Thievessen I, Yarar D, Sabass B, Waterman CM. 2010. A versatile, multi-color total internal reflection fluorescence and spinning disk confocal microscope system for high-resolution live cell imaging. In Live Cell Imaging. A Laboratory Manual. R.D. Goldman, J. Swedlow, D.L. Spector, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 119–138.

Manso AM, Kang SM, Plotnikov SV, Thievessen I, Oh J, Beggs HE, Ross RS. 2009. Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration. Am J Physiol Heart Circ Physiol 296(3):H627-38.

Krüger M, Moser M, Ussar S, Thievessen I, Luber CA, Forner F, Schmidt S, Zanivan S, Fässler R, Mann M. 2008. SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell 134(2):353-64.

Wang HV, Chang LW, Brixius K, Wickström SA, Montanez E, Thievessen I, Schwander M, Müller U, Bloch W, Mayer U, Fässler R. 2008. Integrin-linked kinase stabilizes myotendinous junctions and protects muscle from stress-induced damage. J Cell Biol 180(5):1037-49.

Chu H, Thievessen I, Sixt M, Lämmermann T, Waisman A, Braun A, Noegel AA, Fässler R. 2006. -Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response. Mol Cell Biol 26(5):1817-25.

Thievessen I, Wolter M, Prior A, Seifert HH, Schulz WA. 2005. Hedgehog signaling in normal urothelial cells and in urothelial carcinoma cell lines. J Cell Physiol 203(2):372-7.

Grashoff C, Thievessen I, Lorenz K, Ussar S, Fässler R. 2004. Integrin-linked kinase: integrin's mysterious partner. Curr Opin Cell Biol 16(5):565-71.

Thievessen I, Seifert HH, Swiatkowski S, Florl AR, Schulz WA. 2003. E-cadherin involved in inactivation of WNT/beta-catenin signalling in urothelial carcinoma and normal urothelial cells. Br J Cancer 88(12):1932-8.

Swiatkowski S, Seifert HH, Steinhoff C, Prior A, Thievessen I, Schliess F, Schulz WA. 2003. Activities of MAP-kinase pathways in normal uroepithelial cells and urothelial carcinoma cell lines. Exp Cell Res 282(1):48-57.

Invited Seminars and Talks

University of Zaragoza, Department of Mechanical Engineering, Zaragoza. 06/2014. Invited seminar

53rd Annual Meeting of the American Society for Cell Biology, New Orleans. 12/2013. Short talk

5th European Cell Mechanics Meeting, Obergurgl. 09/2013. Short talk

2nd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg. 09/2012. Short talk

ETH Zurich Swiss Federal Institute of Technology, Laboratory for Biologically Oriented Materials, Zurich. 06/2012. Invited seminar

4th International Symposium Interface Biology of Implants, Warnemuende. 05/2012. Short talk

51st Annual Meeting of the American Society for Cell Biology, Denver. 12/2011. Short talk

National Heart, Lung and Blood Institute, Biochemistry and Biophysics Center, Bethesda. 12/2011. Invited seminar

9th National Heart, Lung and Blood Institute Division of Intramural Research (NHLBI DIR) Scientific Retreat, Cambridge. 04/2011. Short talk

Friedrich-Alexander University Erlangen-Nuernberg, Department of Physics, Biophysics Group, Erlangen. 04/2011. Invited seminar

Max-Planck-Institute of Biochemistry, Department of Molecular Medicine, Martinsried. 09/2010. Invited seminar

National Center for Biotechnology, Department of Cellular and Molecular Biology, Madrid. 09/2010. Invited seminar

Gordon-Kenan Research Seminar “Signaling by Adhesion Receptors”, Waterville. 07/2010. Short talk

National Center for Biotechnology, 17th workshop “Advances in Molecular Biology for Young Investigators”, Madrid. 12/2009. Short talk

Poster Presentations

6th European Cell Mechanics Meeting, Barcelona, 2015

3rd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg, 2015

53rd Annual Meeting of the American Society for Cell Biology, New Orleans. 2013

2nd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg. 2012.

51st Annual Meeting of the American Society for Cell Biology, Denver. 2011

Cell Migration Consortium meeting “Frontiers in Cell Migration & Mechanotransduction”, Bethesda. 2011

9th NHLBI DIR Scientific Retreat, Cambridge. 2011

50th Annual Meeting of the American Society for Cell Biology, Philadelphia. 2010

Gordon Research Conference “Signaling by Adhesion Receptors”, Waterville. 2010

Swedish Association for Cytoskeleton Research meeting “The Cytoskeleton in Development and Pathology”, Stockholm. 2010

8th NHLBI DIR Scientific Retreat, Baltimore. 2010

49th Annual Meeting of the American Society for Cell Biology, San Diego. 2009

National Institutes of Health Research Festival, Bethesda.2009

7th NHLBI DIR Scientific Retreat, Annapolis. 2009

European Molecular Biology Organization workshop “Signals and Mechanics in Directed Cell Migration”, Heidelberg. 2006

European Life Scientist Organisation Meeting, Dresden. 2005

Stefan Münster

 StefanM2010

Physicist, Ph.D. student
Alumnus of the IMPRS Erlangen

email:This email address is being protected from spambots. You need JavaScript enabled to view it.


I have moved on and work now as a ELBE Post-Doc at the Center for Systemsbiology

jointly affiliated with the Max-Planck-Institute for Cell Biology and Genetics and

the Max-Planck-Institute for the Physics of Complex Systems:

MPI-PKS

Office 1B3

Nöthnitzerstr. 38             or:           

01187 Dresden

MPI-CBG

Office 335

Pfotenhauerstr. 108

01307 Dresden

 

 

Experimental techniques

Macro-& microrheological methods, confocal imaging, 3D-traction microscopy, 3D cell tracking, video particle tracking, magnetic tweezers, protein labelling

 

Publications

Münster S., Jawerth L.M., Leslie B.A., Weitz J.I., Fabry B.* and Weitz. D.A.*
Strain history dependence of the nonlinear stress response of fibrin and collagen networks
Proceedings of the National Academy of Science 110(30):12197-12202, (2013). [PDF]

Münster S. and Fabry B.
A Simplified Implementation of the Bubble Analysis of Biopolymer Network Pores
Biophysical Journal 104(12): 2774-5, (2013). [PDF]

Münster S., Jawerth L.M., Fabry B. and Weitz. D.A.
Structure and mechanics of fibrin clots formed under mechanical perturbation
Journal of Thrombosis and Haemostasis 11(3): 557-560, (2013). [PDF]

Krummel A.T. *, Datta, S.S. *, Münster S. and Weitz D.A.
Visualizing Multiphase Flow and Trapped Fluid Configurations in a Model Three-Dimensional Porous Medium
AIChE Journal 59(3):1022–9, (2013). [PDF]


Koch T.M., Münster S., Bonakdar N., Butler J.P. and Fabry B.
3D Traction Forces in Cancer Cell Invasion
PLoS ONE 7(3):e33476, (2012). [PDF]

 

Heit Y.I., Dastouri P., Helm D.L., Pietramaggiori G., Younan G., Erba P., Münster S., Orgill D.P., Scherer S.S.
Foam pore size is a critical interface parameter of suction-based wound healing devices.
Plast Reconstr Surg. 129(3):589-97, (2012).  [PDF]

 

Broedersz C.P., Kasza K.E., Jawerth L.M., Münster S., Weitz D.A. and MacKintosh F.C.
Measurement of nonlinear rheology of cross-linked biopolymer gels
Soft Matter 6:4120-4127,(2010). [PDF]


Jawerth L.M.*, Münster S.*, Vader D.A., Fabry B. and Weitz D.A.
A Blind Spot in Confocal Reflectance Microscopy: The Dependence of Fiber Brightness on Fiber Orientation in Imaging Biopolymer Networks
Biophys J 98(3):L1-L3, (2010). [PDF]

(* authors have contributed equally to this work)


Fabry B., Koch T.M., Münster S., Mierke C.T. and Butler J.P.
Contractile forces during cancer cell invasion
IFMBE Proceedings 25/10:85-86, (2009).


Mickel W.*, Münster S.*, Jawerth L.M., Vader D.A., Weitz D.A., Sheppard A.P., Mecke K., Fabry B. and Schroeder-Turk G.
Robust Pore Size Analysis of Filamentous Networks from 3D Confocal Microscopy
Biophys J 95:6072-80, (2008). [PDF]

(* authors have contributed equally to this work)

 

 

Curriculum Vitae

     2008 – present:

Ph.D. at FAU Erlangen-Nürnberg associated with the International Max-Planck-Research School for Optics and Imaging (IMPRS-OI) at the Max-Planck-Institute for the Science of Light, Erlangen, Germany

     2003 – 2009

Study of biology at FAU Erlangen-Nürnberg

       - Sept. 05

Vordiplom in biology

     2001 – 2008:

Study of physics at FAU Erlangen-Nürnberg

       - July 03

Vordiplom in physics

       - Jan. 08

Diplom (masters degree) in physics

Internships / Research visits

     Jul. 08 – present:

Visiting grad student at Harvard University, DEAS, Weitzlab, Cambridge, MA, USA

     Jan. 08 - Apr. 08:

Visiting student at Harvard University, DEAS, Weitzlab, Cambridge, MA, USA

     Sep. 07:

Sommerakademie from Studienstiftung des Deutschen Volkes – Workgroup for Biomaterials, Görlitz, Germany

     May 07 - Jul. 07:

Visiting student at Harvard School of Public Health, Harvard University, Boston, MA, USA

     Summer 2005:

Summer Student Programme at CERN, Geneva, Switzerland

     Summer 2004:

International Summer Student Program at Gesellschaft für Schwerionenforschung, Darmstadt, Germany

Awards

     Jan. 08:

Ohm-Prize from the Department of Physics, University Erlangen-Nürnberg for 'the best master thesis of the graduation class 2007/2008'

     Jun. 01:

Award for excellent achievement in physics, together with Abitur (highschool degree)

Scholarships / Grants

     Jun. 08 – present:

Fellow of the International Max-Planck-Research-School for Optics and Imaging, Erlangen, Germany

     2004 - 2009:

Scholarship from Studienstiftung des Deutschen Volkes

     Nov 08 – Nov 09:

Full-year Hölderlin-Scholarship from Studienstiftung des Deutschen Volkes to conduct research visit as part of PhD work at Harvard University, Cambridge, MA, USA

     Aug. 08:                    

Travel Grant from DFG to attend The XVth International Congress on Rheology, Monterey, CA, USA

     Jan. 08 – Apr. 08:

Travel Grant from Studienstiftung des Deutschen Volkes to conduct short-term research visit at Harvard University, Cambridge, MA, USA

     Feb. 08:

Travel Grant to attend 'Frontiers in Microrheology', UCLA, Los Angeles, CA, USA

     Jun. 07 - Jul. 07:

Short-term scholarship from DAAD (German Academic Exchange Service) to attend a research visit at Harvard University, Boston, MA, USA



Abstracts / Conferences / Talks

Münster S., Jawerth L., Weitz D. and Fabry B.
The Effect of Large Strain Deformations on the Non-linear Material Properties of Collagen
DPG Frühjahrstagung 2010, Regensburg: Talk

 

Koch T., Münster S., Mierke C., Butler J. and Fabry B.
Strain Energy and its Density Distribution around Invasive and Non-Invasive Carcinoma Cells in 3D Collagen Gels
DPG Frühjahrstagung 2010, Regensburg: Talk

 

Münster S., Jawerth L., Broedersz, C., Weitz D.A. and Fabry B.
Non-linear Mechanical Properties of Collagen Networks under Cyclic Loading
Annual Meeting of the Biophysical Society, 2010, San Francisco, USA: Poster

 

Jawerth L., Münster S. and Weitz D.
Direct Observation of Fiber Dynamics during in Fibrin Networks under Shears
Annual Meeting of the Biophysical Society, 2010, San Francisco, USA: Poster

17.)

Sporer S., Madadi M., Münster S., Mecke K., Arns C., Fabry B. and Schröder-Turk G.
Fiber Networks: Relationship between Effective Elastic Properties and Morphology
DPG Frühjahrstagung 2009, Dresden: Talk

16.)

Münster S., Kollmannsberger P., Koch T.M., Jawerth L.M., Vader D.A. and Fabry B.
Non-linear Rheology Of Collagen Type I Gels Probed On The Length Scale Of A Migrating Cell
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

15.)

Jawerth L., Münster S., Vader D. and Weitz D.
Fiber Dynamics during Strain Stiffening in Stiff Biopolymer Networks
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

14.)

Koch T.M., Münster S., Mierke C.T., Butler J.P. and Fabry B.
Strain Energy of Invasive and Non-Invasive Carcinoma Cells in Three-Dimensional Collagen Gels
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

13.)

Schroeder-Turk G.-E., Mickel W., Münster S., Jawerth L.M., Vader D.A., Weitz D.A., Mecke K.,  and Fabry B.
Robust Pore Size Analysis of Filamentous Networks from 3D Confocal Microscopy
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

12.)

Koch T.M., Münster S., Mierke C.T., Butler J.P. and Fabry B.
Strain Energy of Invasive and Non-Invasive Carcinoma Cells in Three-Dimensional Collagen Gels
48th Annual Meeting of the American Society of Cell Biology, 2008, San Francisco, CA, USA: Poster

11.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Morphological and Mechanical Characterization of Reconstituted Collagen Networks
The XVth International Congress on Rheology, 2008, Monterey, CA, USA: Talk

10.)

Vader D.A., Münster S., Jawerth L.M., Stein A., Fabry B. and Weitz D.A
Large Amplitude Oscillatory Shear in Collagen Rheology Shows Early Strain Stiffening Networks
Journal of Biomechanics, July 2008 (Vol. 41, P S318)

8.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Morphological and Mechanical Characterization of Reconstituted Collagen Type I Networks
DPG Frühjahrstagung, 2008, Berlin: Talk [PDF]

7.)

Koch T.M., Münster S., Mierke C.T., Kollmannsberger P., Butler J.P. and Fabry B.
Strain Energy During Cell Invasion In Three-Dimensional Collagen Gels
DPG Frühjahrstagung, 2008, Berlin: Talk

6.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Mechanical and Morphological Characterization Of Reconstituted Collagen Type I Networks on Different Length Scales
Froniers in Microrheology, 2008, UCLA, Los Angeles, CA, USA: Poster

5.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Mechanical Characterization Of Reconstituted Collagen Type I Networks As A Cell Culture Environment For 3D Traction Microscopy And Cell Invasion Assays
Annual Meeting of the Biophysical Society, 2008, Long Beach, CA, USA: Poster  [PDF]

4.)

Jawerth L.M., Vader D.A., Münster S. and Weitz D.A.
Three Dimensional Structure of Sheared Biopolymer Networks using Confocal Microscopy
Annual Meeting of the Biophysical Society, 2008, Long Beach, CA, USA: Poster

3.)

Münster S., Koch T.M., Kollmannsberger P. and Fabry B.
Force-induced deformations of 3-D collagen gels are consistent with continuum mechanics
2nd European Meeting on Cell Mechanics, 2007, Barcelona: Talk [PDF]

2.)

Koch T.M., Münster S., Mierke C.T., Kollmannsberger P., Butler J.P., Fredberg J.J. and Fabry B.
Strain Energy during Mesenchymal Tumor Cell Invasion in 3-D Collagen Gel
2nd European Meeting on Cell Mechanics, 2007, Barcelona: Talk

1.)

Koch T., Mierke C.T., Paranhos Zitterbart D., Münster S. and Fabry B.
Strain Energy during Tumor Cell Invasion in 3-D Collagen Gels
DPG Frühjahrstagung 2007, Regensburg: Talk [PDF]



Abstract of PhD-Thesis



Theme of Diploma-Thesis

'Elastic strain energy of living cells in a 3-dimensional matrix''

Living cells in a three-dimensional matrix exert substantial forces on their surroundings. The magnitude of these forces are yet unknown. Our group invented a method for measuring these tractions in 3-D by extending the existing method of 2-D traction microscopy to the third dimension. To provide an in vitro environment e.g. for tumor cell invasion we use collagen gels (with a known Young's modulus) with embedded fluorescent marker spheres. As cells migrate into these gels they deform the matrix. The 3-D marker positions are determined with a center-of-mass algorithm applied to microscope images of various focal depths of the gel. To quantify gel contraction two series of images are compared with each other. One, which was taken with the living cell in its native condition and another after addition of a cytotoxin (Cytochalasin-D) which causes the cell to relax. These latter positions represent the 'undeformed' state of the gel after elastic relaxation. Assuming the matrix to be homogeneous and elastic, the stored strain energy can be computed from the deformation the gel underwent. In addition, position and orientation of acting forces can be calculated e.g. by application of finite-element-methods.
In this project, my objective was to verify the methodological approach, to characterize the mechanical properties of the used collagen gel matrices and to conduct experiments to measure traction forces generated in a 3-D environment.
Besides characterizing measurement accuracy and determining the optimal experimental setup, I was able to show that marker displacements in deformed 3-D collagen gels follow the predictions of continuum mechanics for a homogeneous material in the regime of low strains. In order to characterize the hif tsrain behavior, I characterized the rheological properties of the used gels on a macro- as well as a microscale. Furthermore, I was investigating the three dimensional morphology of the reconstituted networks by means of confocal imaging fluorescently labeled gels.

Emmeran Sollner

Emmeran Sollner

Emeran2012Large

Diploma student

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25609
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Project

Diploma Thesis: Mobile Observation Platforms (Atka Bay Penguin Observatory)


 

Curriculum Vitae

 

Publications

Whyte Lab

We are interested in developing and using novel microfluidic and biophotonic tools to measure the nuclear properties of living cells. Our research focusses on using optical trapping to study the structure of the cell nucleus with higer precision, and on using optical and microfluidic methods for measuring the mechanical properties of the nucleus to understand how genetic and expression level changes can alter the physical properties of the nucleus.

Single-Cell Tomography

When trying to understand the 3D structure and nature of objects, it is often easier to look at the object from different sides. With microscopic objects it becomes more difficult to hold and accurately rotate the objects, and it is made even more difficult when the object is a fragile, living cell. We have developed several methods for doing exactly this - we use lasers to be able to delicately hold the cell in place, without physically touching it, and then we can continously rotate the cell by applying extra, controlled forces.

Optofluidic Cell Rotator - The microfluidic water mill

Schematic of the Optofluidic Cell RotatorOne method we have developed for rotating a cell is based on a tiny version of a water mill: We hold the the cell in our optical trap which uses lasers to avoid physically touching

Flow velocity around a cell trapped in the Optofluidic Flow Rotator

Simulation of the velocity flow field of liquid passing around a cell trapped in the Optofluidic Flow Rotator. Colours represent the speed of the liquid as it flows from right to left

the cell and then flow a microfluidic river past it.

The flowing liquid pulls the cell around and causes it to rotate around the laser beam. The faster we flow the liquid, the faster the cell rotates.

 

 The system works due to holding the cell in the optimum position in the microfluidic flow. When liquid flows next to the walls, it is slowed down by the friction, while the liquid in the middle flows much faster - creating the parabolic flow velocity profile shown on the right. If the cell is positioned away from the middle of the flow, then there will be faster liquid flowing over the top of the cell, than below the cell, leading to a rotational torque.

 

Click below to see a short movie describing the system and demonstrating the rotation.

Click to download in FLV format (4.94MB)

The system works with almost any type of cell and can be used with many different microscopy techniques. By rotating the cell, it is also possilbe to improve the resolution of the final image and bypass the inherent low z-resolution when normally measuring 3D images.

 

 
 

Dynamic Fibre Optic Spanner - The micro-paper windmill

Another technique for rotating delicate cells is based on the paper windmill toys which can be spun around just by blowing across them. Cells prefer to be swimming in liquid so we can't use air to rotate them. We once again use the power of laser light to to gently 'blow' across the top (and bottom) of the cell while it is held in the optical trap.

gwhyte-DFOS-schemeWe have developed a novel device which can move the laser light to rotate the cell around almost any angle, allowing you to see the cell from all sides.

It works by pushing optical fibres, which carry the laser light to trap, using air pressure. The device contains 'fibre pads' which can be inflated, causing them to expand and push the fibre away. By surrounding the optical fibre with these pads it is possible to rotate the cell around the optical axis as well as perpendicular axes - allowing fibre spanners to be used for optical tomography for the first time.

 

Nuclear Mechanics

The mechanical properties of the cell nucleus is still not fully understood. The dense packing of all the DNA into a very compact space results in the nucleus being very stiff, however changes in the nucleus as the cell divides, differentiates or is affected by disease, can alter this stiffness. By developing tools to measure the mechanical properties of the nucleus, we hope to understand what underlies the change in stiffness and create more accurate and usable diagnostic tests.

The Optical Stretcher

The Optical Stretcher (OS) is an established technique for measuring the mechanical properties of cells using laser light. As the light passes through a cell, the light transmits forces to the surface of the cell, which can cause it to deform. This deformation can be measured with normal microscopy techniques and gives a measure of total stiffness of the whole cell.

By measuring the whole-cell deformation for different nuclear conditions we can assess how the small changes in the nucleus can lead to large changes in the overall cell mechanics.

Janina Lange

PhD student

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25609;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Projects

Pore size distributions in collagen networks (2011)

Thermal Noise Imaging: observing the topology of collagen networks (2012), cooperation with Prof. E.-L. Florin

Collective cell migration (2012-2013), cooperation with PD Pamela Strissel, PhD

Cell rheology measurements with microfluidic devices (2013-...), cooperation with Prof. Graeme Whyte

 

Curriculum Vitae

FAU Erlangen-Nuremberg:

Physics (B.Sc.) , 2011

Physics (M.Sc.) ,  2013

PhD, (2013-...)

 

Conferences

Physics of Cancer (3rd Annual Meeting), Leipzig, Germany, 2012/11/01-2012/11/03

Janina R. Lange, Claus Metzner, Julian Steinwachs, Pamela Strissel, Ben Fabry

3D topology of tumor cell colonies on a 2D substrate

 

Biophysical Society 57th Meeting, Philadelphia, USA, 2013/02/02-2013/02/06

Tobias F. Bartsch, Martin D. Kochanczyk, Janina R. Lange, Ernst-Ludwig Florin

Three-dimensional thermal noise imaging of collagen networks

 

Annual Meeting of the German Physical Society (DPG), Regensburg, Germany,  2013/03/11-2013/03/15

Janina R. Lange, Claus Metzner, Patrick Krauss, Pamela Strissel, Ben Fabry

The role of collective cell migration during tumor growth

Patrick Krauss, Claus Metzner, Janina Lange, Ben Fabry

Persistent motion in the crowd - the role of superdiffusivity in cell colony dynamics

 

Physics of Emergent Behavior (IOP), Brighton, GB, 2013/06/23-2013/06/26:

Janina R. Lange, Claus Metzner, Patrick Krauss, Pamela Strissel, Ben Fabry

Collective migration during the growth of epithelial and mesenchymal cell colonies

Poster Prize

Patrick Krauss, Claus Metzner, Janina R. Lange, Ben Fabry

Emergence of cell colony dynamics from proliferating particles with Lennard-Jones like interactions

 

5th European Cell Mechanics Meeting, Obergurgel, Austria, 2013/09/28-2013/10/02:

Janina R. Lange, Claus Metzner, Patrick Krauss, Pamela Strissel, Ben Fabry

Collective Migration during the Growth of mesenchymal and epithelial cell colonies

 

Biophysical Society 58th Meeting, San Francisco, USA, 2014/02/15-2014/02/19:

Janina R. Lange, Michael Haug, Thorsten Kolb, Sahradha Albert, Graeme Whyte, Ben Fabry

Nuclear deformability is critically dependent on Lamin A/B

 

Physics of Cancer, (5rd Annual Meeting), Leipzig, Germany, 2014/10/02-2014/10/05:

Janina R. Lange, Thorsten Kolb, Jonas Hallmen, Graeme Whyte, Ben Fabry

Cell deformability during passage through micron-scale constrictions

 

6th European Cell Mechanics Meeting, Barcelona, Spain, 2015/05/13-2015/05/15:

Janina R. Lange, Julian Steinwachs, Thorsten Kolb, Lena A. Lautscham, Irina Harder, Graeme Whyte, Ben Fabry

Microconstriction arrays for high throughput quantitative measurements of cell mechanical properties

 

Biophysical Society 60th Meeting, Los Angeles, USA, 2016/02/27-2016/03/02:

Janina R. Lange, Julian Steinwachs, Claus Metzner, Graeme Whyte, Ben Fabry

Cell mechanical properties measured with micron-scale constrictions: influence of pressure, strain, and culture conditions

 

Annual meeting of the German biophysical society, Erlangen, Germany, 2016/10/04-2016/10/06:

Janina R. Lange, Claus Metzner, Sebastian Richter, Werner Schneider, Monika Spermann, Thorsten Kolb, Graeme Whyte, Ben Fabry

Unbiased high precision cell mechanical measurements with microconstrictions

 

Physics of Cancer, (7th Annual Meeting), Leipzig, Germany, 2016/10/04-2016/10/06:

Janina R. Lange, Claus Metzner, Sebastian Richter, Werner Schneider, Monika Spermann, Thorsten Kolb, Graeme Whyte, Ben Fabry

Unbiased high precision cell mechanical measurements with microconstrictions

 

 

Publications

Krauss P., Metzer C., Lange J.R., Lang N., Fabry B.
Parameter-Free Binarization and Skeletonization of Fiber Networks from Confocal Image Stacks
PLoS ONE 7:e36575, 2012 (PDF)

 

Lange J.R., Fabry B.

Cell and tissue mechanics in cell migration

Exp Cell Res 319: 2418–2423, 2013 (PDF)

 

Lange J.R., Auernheimer V., Strissel P., Goldmann W.H.

Influence of focal adhesion kinase on the mechanical behavior of cell populations

BBRC 436: 246-251, 2013 (PDF)

 

Lang N., Münster S., Metzner C., Krauss P., Lange J.R., Schürmann S., Aifantis K.E., Friedrich O, Fabry B.

Estimating the 3D pore size distribution of biopolymer networks from directionally biased data

Biophys. J. 105: 1967-1975, 2013 (PDF)

 

Lange, J.R., Steinwachs, J. Kolb, T. Lautscham, L.A., Harder, I. Whyte, G., Fabry, B.

Microconstriction arrays for high-throughput quantitative measurements of cell mechanical properties

 Biophys J. 109: 26–34, 2015 (PDF)

 

Lautscham L.A., Kämmerer C., Lange J.R., Kolb T., Mark C., Schilling A., Strissel P., Strick R., Gluth C., Rowat A., Metzner C., Fabry B.

Mechanical cell properties that influence migration in confined 3D environments

Biophys J 109:900-913, 2015 (PDF)

 

Bartsch T.F., Kochanczyk M.D., Lissek E.N., Lange J.R., Florin E.-L.

Nanoscopic imaging of thick heterogeneous soft-matter structures in aqueous solution

Nat. Commun. 7:12729 doi: 10.1038/ncomms12729,  2016 (PDF)

 

Lange J.R., Goldmann, W.H., Alonso J.L.

Influence of αvβ3 integrin on the mechanical properties and the morphology of M21 and K562 cells

BBRC (2016), 478:1280-5 (PDF)

 

Lange J.R., Metzner C., Richter S., Schneider W. Spermann M.,  Kolb T., Whyte G., Fabry B.

Unbiased high precision cell mechanical measurements with  microconstrictions

Biophys J , in press (PDF)

 

Marina Spörrer

D1000010

PhD student

Room 02.076

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

Curriculum Vitae

FAU Erlangen-Nuremberg:

Biology (B.Sc.) , 2012

Biology (M.Sc.) ,  2014

PhD. (2014-...)

 

Conferences

Biomechanical behavior of myotubes with a desmin R350P mutation
Spörrer M., Winter L., Schröder R., Fabry B., Goldmann W.H.
Talk, 6th European Cell Mechanics Meeting, Barcelona, 2015

 

Publications

Bonakdar N., Schilling A., Lennert P., Spörrer M., Gerum R.C., Alonso J.L. and Goldmann W.H.
Measuring mechanical properties in cells: three easy methods for biologists
Cell Biology International 38: 1227-1232, 2014 (PDF)

Bonakdar N., Schilling A., Spörrer M., Lennert P., Mainka A., Winter L., Walko G., Wiche G., Fabry B. and Goldmann W.H.
Determining the mechanical properties of plectin in mouse myoblasts and keratinocytes
Exp Cell Res 331: 331-337, 2015, doi: 10.1016/j.yexcr.2014.10.001 (PDF)

Ben Fabry

Prof. Dr. Ben Fabry
Director, Center for Medical Physics and Technology
Biophysics Group
Room 02.072
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25610
fax: (+49) (0)9131-85-25601

This email address is being protected from spambots. You need JavaScript enabled to view it.


Research interests:

Our laboratory studies the mechanical behavior of cells, including cell adhesion, spreading, deformation, motion, migration, invasion, stiffness, viscosity, force generation and mechano-transduction. These mechanical processes are essential for many cellular functions. Abnormal cell mechanical behavior is often associated with severe disorders such as cancer or cardiovascular diseases. We collaborate with research groups worldwide to develop novel technologies that enable us to measure or apply small forces (in the pico-Newton range) and deformations (in the low nano-meter range) that are relevant for cell mechanical functions. We use these technologies to study the behavior of cells on nano-structured biomaterials, cancer cell migration in 3-dimensional biopolymer networks, or the biochemistry of adhesion proteins.


Curriculum Vitae

Ben Fabry studied Electrical Engineering at the Technical University of Dresden, Germany. In 1991 he joined the R&D group of the Anesthesiology and Intensive Care Medicine division at Draegerwerk AG, Luebeck, Germany, and subsequently the Department of Clinical Physiology at the University of Basel, Switzerland. After receiving his doctorate degree in Electrical Engineering in 1995, he was a research assistant, and after 1999 a research associate at the Physiology Program, Harvard School of Public Health, Boston, MA, where he worked on smooth muscle physiology and cellular biomechanics. Since 2003, Ben Fabry has been Professor for Medical Physics and Technology at the University of Erlangen-Nuremberg, Germany, and since 2005 he has been the administrative head of the Center for Medical Physics and Technology at the University.

The Erlangen Biophysics Group

Marina
ben
Ben Fabry
Biophysics
Wolfgang Goldmann
Wolfgang Goldmann
Biochemistry
Heike Mertin
Heike Mertin
Secretary
im1266ws
Ԝemer Schnеidеr
Technician
Astrid Mainka
Astrid Mainka
biol. laboratory
Claus Metzner
Claus Metzner
Theoretical Physics
Ingo Thievessen
Ingo Thievessen
Cell Biology
Daniel Paranhos Zitterbart
Daniel Zitterbart Post-Doc
SebastianRichter2013
Sebastian Richter
PhD student
Richard Gerum
Richard Gerum
PhD student
Janina Lange
Janina Lange
PhD student
Marina Spörrer
Marina Spörrer
PhD student
Christoph Mark
Christohp Mark
PhD student

 

Alumni

Claire Amado

  2016    
Fumina Yoshimoto   2016   Okayama University, Japan

20151016 103950 cut-pola mini

Breff Whitehead

  2015-2016   Cape Town

20151026 132227 cut mini

Laryn Wessels

  2015-2016   Cape Town
Camille Metz   2015   École Polytechnique Paris
Julian Steinwachs
Julian Steinwachs
PhD student 2011-2015 link to website Programmer at Redheads Software, Erlangen
VeraA2012
Vera Auernheimer
PhD student 2011-2015 link to website tba
Lena Lautscham
Lena Lautscham
PhD student 2011-2015  link to website tba

Nadine Lang
Nadine Lang

PhD student 2010-2014  link to website Scientific project coordinator, Fraunhofer IIS Erlangen

Jaroslav Branis

  2014   Charles University Prague

Czilla Pataki

  2013   Charles University Prague
AchimS2012
Achim Schilling
Masters student 2012-2013 This email address is being protected from spambots. You need JavaScript enabled to view it. Experimental Otolaryngology Lab, Universität Erlangen-Nürnberg

ChristianW2012
Christian Weis

PhD student 2010-2013 link to website Universitätsklinikum Heidelberg, Diagnostische und Interventionelle Radiologie
Peta Bradbury
Peta Bradbury
Visiting Researcher 2013  http://www.kidsresearch.org.au/research/focal-adhesion-biology/members.php Children's Hospital Westmead, The University of Sydney

Navid Bonakdar
Navid Bonakdar

PhD student 2009-2013  link to website MPI for the Physics of Light, Erlangen

StefanM2010
Stefan Münster

PhD student 2009-2013 link to website Center for Systems Biology, Dresden (MPI for Cell Biology and Genetics + MPI for the Physics of Complex Systems)
Johannes Pauli post-doc 2003-2005 This email address is being protected from spambots. You need JavaScript enabled to view it. Siemens Medical Solutions
Philip Kollmannsberger PhD student 2004-2009 This email address is being protected from spambots. You need JavaScript enabled to view it. Laboratory of Applied Mechanobiology, ETH Zürich
Carina Raupach PhD student 2004-2008 This email address is being protected from spambots. You need JavaScript enabled to view it. Areva Nuclear Instrumentation

Tuli Dey
Tuli Dey

post-doc 2009-2011 This email address is being protected from spambots. You need JavaScript enabled to view it. back in India on the way to Singapore

Andreas Schönborn
Andreas Schönborn

Diploma Student 2011 unknown unknown

Michael KuhnMichael Kuhn

Diploma Student 2011 unknown unknown

Radoslav Janostiak

  2011   Charles University Prague

Masahiro Tsuchiya

2011     Japan
Caroline Gluth PhD student 2012 This email address is being protected from spambots. You need JavaScript enabled to view it. Institut für Phonetik und Sprachverarbeitung,LMU München
Corey Yuhung Lin   2011    
Max Dietrich Diploma Student 2011 This email address is being protected from spambots. You need JavaScript enabled to view it. Willamed Medizintechnik GmbH
Gerold Diez    2006-2009 This email address is being protected from spambots. You need JavaScript enabled to view it. Virion-Serion GmbH, Würzburg

CarlosB2012

Carlos Borau

Intern PhD 2012 This email address is being protected from spambots. You need JavaScript enabled to view it. University of Zaragoza, Spain
Leila Jaafar PhD student  2007-2010 This email address is being protected from spambots. You need JavaScript enabled to view it. AREVA NP GmbH, Erlangen
Thorsten Koch PhD student 2011   COMSOL Multiphysics GmbH
Dieter Freitag Diploma student 2008 This email address is being protected from spambots. You need JavaScript enabled to view it. Siemens AG, Amberg
         
James Smith  post-doc 2006 This email address is being protected from spambots. You need JavaScript enabled to view it. Jacobs University Bremen
Emeran2012
Emmeran Sollner
Diploma student 2012 This email address is being protected from spambots. You need JavaScript enabled to view it.