Stefan Münster

 StefanM2010

Physicist, Ph.D. student
Alumnus of the IMPRS Erlangen

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I have moved on and work now as a ELBE Post-Doc at the Center for Systemsbiology

jointly affiliated with the Max-Planck-Institute for Cell Biology and Genetics and

the Max-Planck-Institute for the Physics of Complex Systems:

MPI-PKS

Office 1B3

Nöthnitzerstr. 38             or:           

01187 Dresden

MPI-CBG

Office 335

Pfotenhauerstr. 108

01307 Dresden

 

 

Experimental techniques

Macro-& microrheological methods, confocal imaging, 3D-traction microscopy, 3D cell tracking, video particle tracking, magnetic tweezers, protein labelling

 

Publications

Münster S., Jawerth L.M., Leslie B.A., Weitz J.I., Fabry B.* and Weitz. D.A.*
Strain history dependence of the nonlinear stress response of fibrin and collagen networks
Proceedings of the National Academy of Science 110(30):12197-12202, (2013). [PDF]

Münster S. and Fabry B.
A Simplified Implementation of the Bubble Analysis of Biopolymer Network Pores
Biophysical Journal 104(12): 2774-5, (2013). [PDF]

Münster S., Jawerth L.M., Fabry B. and Weitz. D.A.
Structure and mechanics of fibrin clots formed under mechanical perturbation
Journal of Thrombosis and Haemostasis 11(3): 557-560, (2013). [PDF]

Krummel A.T. *, Datta, S.S. *, Münster S. and Weitz D.A.
Visualizing Multiphase Flow and Trapped Fluid Configurations in a Model Three-Dimensional Porous Medium
AIChE Journal 59(3):1022–9, (2013). [PDF]


Koch T.M., Münster S., Bonakdar N., Butler J.P. and Fabry B.
3D Traction Forces in Cancer Cell Invasion
PLoS ONE 7(3):e33476, (2012). [PDF]

 

Heit Y.I., Dastouri P., Helm D.L., Pietramaggiori G., Younan G., Erba P., Münster S., Orgill D.P., Scherer S.S.
Foam pore size is a critical interface parameter of suction-based wound healing devices.
Plast Reconstr Surg. 129(3):589-97, (2012).  [PDF]

 

Broedersz C.P., Kasza K.E., Jawerth L.M., Münster S., Weitz D.A. and MacKintosh F.C.
Measurement of nonlinear rheology of cross-linked biopolymer gels
Soft Matter 6:4120-4127,(2010). [PDF]


Jawerth L.M.*, Münster S.*, Vader D.A., Fabry B. and Weitz D.A.
A Blind Spot in Confocal Reflectance Microscopy: The Dependence of Fiber Brightness on Fiber Orientation in Imaging Biopolymer Networks
Biophys J 98(3):L1-L3, (2010). [PDF]

(* authors have contributed equally to this work)


Fabry B., Koch T.M., Münster S., Mierke C.T. and Butler J.P.
Contractile forces during cancer cell invasion
IFMBE Proceedings 25/10:85-86, (2009).


Mickel W.*, Münster S.*, Jawerth L.M., Vader D.A., Weitz D.A., Sheppard A.P., Mecke K., Fabry B. and Schroeder-Turk G.
Robust Pore Size Analysis of Filamentous Networks from 3D Confocal Microscopy
Biophys J 95:6072-80, (2008). [PDF]

(* authors have contributed equally to this work)

 

 

Curriculum Vitae

     2008 – present:

Ph.D. at FAU Erlangen-Nürnberg associated with the International Max-Planck-Research School for Optics and Imaging (IMPRS-OI) at the Max-Planck-Institute for the Science of Light, Erlangen, Germany

     2003 – 2009

Study of biology at FAU Erlangen-Nürnberg

       - Sept. 05

Vordiplom in biology

     2001 – 2008:

Study of physics at FAU Erlangen-Nürnberg

       - July 03

Vordiplom in physics

       - Jan. 08

Diplom (masters degree) in physics

Internships / Research visits

     Jul. 08 – present:

Visiting grad student at Harvard University, DEAS, Weitzlab, Cambridge, MA, USA

     Jan. 08 - Apr. 08:

Visiting student at Harvard University, DEAS, Weitzlab, Cambridge, MA, USA

     Sep. 07:

Sommerakademie from Studienstiftung des Deutschen Volkes – Workgroup for Biomaterials, Görlitz, Germany

     May 07 - Jul. 07:

Visiting student at Harvard School of Public Health, Harvard University, Boston, MA, USA

     Summer 2005:

Summer Student Programme at CERN, Geneva, Switzerland

     Summer 2004:

International Summer Student Program at Gesellschaft für Schwerionenforschung, Darmstadt, Germany

Awards

     Jan. 08:

Ohm-Prize from the Department of Physics, University Erlangen-Nürnberg for 'the best master thesis of the graduation class 2007/2008'

     Jun. 01:

Award for excellent achievement in physics, together with Abitur (highschool degree)

Scholarships / Grants

     Jun. 08 – present:

Fellow of the International Max-Planck-Research-School for Optics and Imaging, Erlangen, Germany

     2004 - 2009:

Scholarship from Studienstiftung des Deutschen Volkes

     Nov 08 – Nov 09:

Full-year Hölderlin-Scholarship from Studienstiftung des Deutschen Volkes to conduct research visit as part of PhD work at Harvard University, Cambridge, MA, USA

     Aug. 08:                    

Travel Grant from DFG to attend The XVth International Congress on Rheology, Monterey, CA, USA

     Jan. 08 – Apr. 08:

Travel Grant from Studienstiftung des Deutschen Volkes to conduct short-term research visit at Harvard University, Cambridge, MA, USA

     Feb. 08:

Travel Grant to attend 'Frontiers in Microrheology', UCLA, Los Angeles, CA, USA

     Jun. 07 - Jul. 07:

Short-term scholarship from DAAD (German Academic Exchange Service) to attend a research visit at Harvard University, Boston, MA, USA



Abstracts / Conferences / Talks

Münster S., Jawerth L., Weitz D. and Fabry B.
The Effect of Large Strain Deformations on the Non-linear Material Properties of Collagen
DPG Frühjahrstagung 2010, Regensburg: Talk

 

Koch T., Münster S., Mierke C., Butler J. and Fabry B.
Strain Energy and its Density Distribution around Invasive and Non-Invasive Carcinoma Cells in 3D Collagen Gels
DPG Frühjahrstagung 2010, Regensburg: Talk

 

Münster S., Jawerth L., Broedersz, C., Weitz D.A. and Fabry B.
Non-linear Mechanical Properties of Collagen Networks under Cyclic Loading
Annual Meeting of the Biophysical Society, 2010, San Francisco, USA: Poster

 

Jawerth L., Münster S. and Weitz D.
Direct Observation of Fiber Dynamics during in Fibrin Networks under Shears
Annual Meeting of the Biophysical Society, 2010, San Francisco, USA: Poster

17.)

Sporer S., Madadi M., Münster S., Mecke K., Arns C., Fabry B. and Schröder-Turk G.
Fiber Networks: Relationship between Effective Elastic Properties and Morphology
DPG Frühjahrstagung 2009, Dresden: Talk

16.)

Münster S., Kollmannsberger P., Koch T.M., Jawerth L.M., Vader D.A. and Fabry B.
Non-linear Rheology Of Collagen Type I Gels Probed On The Length Scale Of A Migrating Cell
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

15.)

Jawerth L., Münster S., Vader D. and Weitz D.
Fiber Dynamics during Strain Stiffening in Stiff Biopolymer Networks
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

14.)

Koch T.M., Münster S., Mierke C.T., Butler J.P. and Fabry B.
Strain Energy of Invasive and Non-Invasive Carcinoma Cells in Three-Dimensional Collagen Gels
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

13.)

Schroeder-Turk G.-E., Mickel W., Münster S., Jawerth L.M., Vader D.A., Weitz D.A., Mecke K.,  and Fabry B.
Robust Pore Size Analysis of Filamentous Networks from 3D Confocal Microscopy
Annual Meeting of the Biophysical Society, 2009, Boston, MA, USA: Poster

12.)

Koch T.M., Münster S., Mierke C.T., Butler J.P. and Fabry B.
Strain Energy of Invasive and Non-Invasive Carcinoma Cells in Three-Dimensional Collagen Gels
48th Annual Meeting of the American Society of Cell Biology, 2008, San Francisco, CA, USA: Poster

11.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Morphological and Mechanical Characterization of Reconstituted Collagen Networks
The XVth International Congress on Rheology, 2008, Monterey, CA, USA: Talk

10.)

Vader D.A., Münster S., Jawerth L.M., Stein A., Fabry B. and Weitz D.A
Large Amplitude Oscillatory Shear in Collagen Rheology Shows Early Strain Stiffening Networks
Journal of Biomechanics, July 2008 (Vol. 41, P S318)

8.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Morphological and Mechanical Characterization of Reconstituted Collagen Type I Networks
DPG Frühjahrstagung, 2008, Berlin: Talk [PDF]

7.)

Koch T.M., Münster S., Mierke C.T., Kollmannsberger P., Butler J.P. and Fabry B.
Strain Energy During Cell Invasion In Three-Dimensional Collagen Gels
DPG Frühjahrstagung, 2008, Berlin: Talk

6.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Mechanical and Morphological Characterization Of Reconstituted Collagen Type I Networks on Different Length Scales
Froniers in Microrheology, 2008, UCLA, Los Angeles, CA, USA: Poster

5.)

Münster S., Koch T.M., Kollmannsberger P., Jawerth L.M., Vader D.A., Schroeder-Turk G. and Fabry B.
Mechanical Characterization Of Reconstituted Collagen Type I Networks As A Cell Culture Environment For 3D Traction Microscopy And Cell Invasion Assays
Annual Meeting of the Biophysical Society, 2008, Long Beach, CA, USA: Poster  [PDF]

4.)

Jawerth L.M., Vader D.A., Münster S. and Weitz D.A.
Three Dimensional Structure of Sheared Biopolymer Networks using Confocal Microscopy
Annual Meeting of the Biophysical Society, 2008, Long Beach, CA, USA: Poster

3.)

Münster S., Koch T.M., Kollmannsberger P. and Fabry B.
Force-induced deformations of 3-D collagen gels are consistent with continuum mechanics
2nd European Meeting on Cell Mechanics, 2007, Barcelona: Talk [PDF]

2.)

Koch T.M., Münster S., Mierke C.T., Kollmannsberger P., Butler J.P., Fredberg J.J. and Fabry B.
Strain Energy during Mesenchymal Tumor Cell Invasion in 3-D Collagen Gel
2nd European Meeting on Cell Mechanics, 2007, Barcelona: Talk

1.)

Koch T., Mierke C.T., Paranhos Zitterbart D., Münster S. and Fabry B.
Strain Energy during Tumor Cell Invasion in 3-D Collagen Gels
DPG Frühjahrstagung 2007, Regensburg: Talk [PDF]



Abstract of PhD-Thesis



Theme of Diploma-Thesis

'Elastic strain energy of living cells in a 3-dimensional matrix''

Living cells in a three-dimensional matrix exert substantial forces on their surroundings. The magnitude of these forces are yet unknown. Our group invented a method for measuring these tractions in 3-D by extending the existing method of 2-D traction microscopy to the third dimension. To provide an in vitro environment e.g. for tumor cell invasion we use collagen gels (with a known Young's modulus) with embedded fluorescent marker spheres. As cells migrate into these gels they deform the matrix. The 3-D marker positions are determined with a center-of-mass algorithm applied to microscope images of various focal depths of the gel. To quantify gel contraction two series of images are compared with each other. One, which was taken with the living cell in its native condition and another after addition of a cytotoxin (Cytochalasin-D) which causes the cell to relax. These latter positions represent the 'undeformed' state of the gel after elastic relaxation. Assuming the matrix to be homogeneous and elastic, the stored strain energy can be computed from the deformation the gel underwent. In addition, position and orientation of acting forces can be calculated e.g. by application of finite-element-methods.
In this project, my objective was to verify the methodological approach, to characterize the mechanical properties of the used collagen gel matrices and to conduct experiments to measure traction forces generated in a 3-D environment.
Besides characterizing measurement accuracy and determining the optimal experimental setup, I was able to show that marker displacements in deformed 3-D collagen gels follow the predictions of continuum mechanics for a homogeneous material in the regime of low strains. In order to characterize the hif tsrain behavior, I characterized the rheological properties of the used gels on a macro- as well as a microscale. Furthermore, I was investigating the three dimensional morphology of the reconstituted networks by means of confocal imaging fluorescently labeled gels.