Ingo Thievessen

Post-Doc/Projectleader

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25606
fax: (+49) (0)9131-85-25601
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Project

The role of vinculin in coordinating cellular morphodynamics, force generation and ECM remodeling during cell migration in 2D and 3D environments

Coordinated cell migration is critical for tissue morphogenesis and homeostasis, and requires the integration of actin cytoskeleton based force generation and cell adhesion to the extracellular matrix (ECM) through integrin adhesion receptors. How force generation and ECM-adhesion affect cell motility has mainly been studied using two-dimensional (2D) cell culture substrates, which cannot recapitulate the mechanics of cell migration in vivo, where cells have to overcome the steric hindrance of the ECM to move forward. Three-dimensional (3D) matrices of collagen, fibrin, or cell-derived matrices are being used more frequently. However, how traction force generation, ECM-adhesion and physical parameters of the ECM influence cell migration in 3D environments still remains unclear, and can vary fundamentally between different cell types.

The coordinated migration of cells in vivo is often associated with a remodeling of the ECM, which modulates haptic and mechanical guidance cues for the cells. In turn, excessive ECM-remodeling may promote cell motility in pathological conditions such as cancer metastasis. Cell motion and ECM-remodeling both depend on cellular traction forces, allowing cells to move forward and apply tension to remodel the ECM, suggesting a role for ECM-traction force generation in coordinating cell motion and ECM-remodeling. It remains unknown, however, which cytoskeleton and adhesion proteins are required to coordinate the remodeling of complex 3D matrices.

The adhesion protein vinculin is present in cell-cell junctions and in focal adhesions (FA), where it links filamentous (F-) actin to the integrin-binding protein talin to transmit forces between actin cytoskeleton and ECM. Vinculin reduces cell migration on 2D substrates. In addition, vinculin expression is reduced in a number of human tumors, and cancer cells overexpressing vinculin show reduced metastatic capacity when injected into nude mice, suggesting that vinculin may also inhibit cell migration in vivo. However, vinculin seems to affect 3D cell migration fundamentally different from 2D cell migration, as it promotes the invasion of fibroblasts into 3D collagen gels. It remains unclear though, how vinculin facilitates cell invasion into collagen. The collagen invasiveness of different cancer cells correlated with the strain energy amounts generated by the cells, with the strain energy field anisotropy, and cell shape anisotropy . Vinculin affects both cell morphology and force generation on 2D substrates, suggesting that vinculin may also promote collagen invasion by modulating force generation and cell shape. It is not known, however, how vinculin affects cell morphodynamics, traction force generation, or ECM remodeling in complex 3D environments.

We characterize the effects of vinculin gene disruption on cell migration, morphodynamics, force generation and ECM-remodeling of primary murine embryonic fibroblast (MEF) on 2D substrates and in 3D collagen I culture.

Current Position

Scientific Assistant

Friedrich-Alexander-University Erlangen-Nuremberg.

Department of Physics, Biophysics Group

Education

Dr. rer. nat.,summa cum laude, 10/2002 - 04/2007. Mouse Genetics/Cell Biology. Max-Planck-Institute of Biochemistry, Martinsried/Heinrich-Heine-University Duesseldorf, Germany. Prof. Dr. med. Reinhard Faessler

Dipl. Biol., 10/1996 - 04/2002. Cell Biology/Biochemistry/Physics. Heinrich-Heine-University Duesseldorf, Germany. Prof. Dr. rer. nat. Wolfgang Schulz

Research Experience

Scientific Assistant, 03/2012 - present. Friedrich-Alexander University Erlangen-Nuremberg, Chair for Biophysics, Biophysics Group. Prof. Dr. Ben Fabry. Project: The role of vinculin in morphodynamics and force generation of primary murine embryonic fibroblasts and endothelial cells in 3D collagen matrices and in vivo

Postdoctoral Fellow, 09/2007 - 02/2012. National, Heart, Lung and Blood Institute/National Institutes of Health, Cell Biology and Physiology Center, Laboratory of Cell and Tissue Morphodynamics. Prof. Dr. Clare M. Waterman, Ph.D.. Project: The role of vinculin in actin based force generation by focal adhesions in migrating primary murine embryonic fibroblasts

Postdoctoral Fellow, 05/2007 - 08/2007. The Scripps Research Institute, Department of Cell Biology, Cell Motility Group. Prof. Dr. Clare M. Waterman, Ph.D.. Project: The role of vinculin in actin based force generation by focal adhesions in migrating primary murine embryonic fibroblasts

Doctoral Student, 10/2002 - 04/2007. Department of Molecular Medicine, Max-Planck-Institute of Biochemistry, Martinsried. Prof. Dr. med. Reinhard Faessler. Project: Functional analysis of focal adhesion proteins of the parvin family by knockout in the mouse

Diploma Student, 03/2001 - 03/2002. Research Laboratory of the Urological Clinic, Heinrich-Heine-University Duesseldorf. Prof. Dr. rer. nat. Wolfgang A. Schulz. Project: Basic and inducible activities of the Wnt- and Hedgehog-signaltransduction pathways in normal and transformed uroepithelial cells

Undergraduate Student, 03/2000 - 12/2000. Department of Cell Biology, Institute of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine-University Duesseldorf. Prof. Dr. rer. nat. Jochen D´Haese. Project: Identification of flea populations by sequence analysis of the internal ribosomal spacer region 1

Teaching Experience

Course Assistant “Biomedical Imaging”, Department of Physics, Biophysics Group, Friedrich-Alexander University Erlangen-Nuermberg. 2014

Course Assistant “Experimental Physics”, Department of Physics, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg. 2012/2013

Teaching Assistant “Physiology Course”, Marine Biological Laboratory, Woods Hole. 2011

Teaching Assistant “Optical Microscopy and Imaging in the Biomedical Sciences”, Marine Biological Laboratory, Woods Hole. 2007

Course Assistant “Animal Morphology”, Institute of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine-University Duesseldorf. 2000

Training, Activities, Memberships

Projektleiter nach GenTSV, Friedrich-Alexander University Erlangen-Nuermberg, Chair for Physical and Medical Technology. 2013

Attendance at laboratory safety/operational courses, Projektleiter nach GenTSV, Sondermuell, Brandgefahren. 2013

National Heart, Lung and Blood Institute Fellows Advisory Committee Member, National Heart, Lung and Blood Institute/National Institutes of Health, Bethesda. 2010-2012

American Society for Cell Biology Member, since 2007

Fellowships and Awards

Travel Grant, 53rd Annual Meeting of the American Society for Cell Biology, New Orleans, 2013

Travel Grant, Swedish Association for Cytoskeleton Research meeting “The Cytoskeleton in Development and Pathology”, Federation of the Societies of Biochemistry and Molecular Biology (FEBS)”. 2010

Fellows Award for Research Excellence 2010, National Institutes of Health, Bethesda. 2009

Publications

Thievessen I, Fakhri N, Steinwachs J, Kraus V, McIsaac RS, Gao L, Chen B, Betzig E, Oldenbourg R, Waterman CM, Fabry B. 2015. Vinculin is required for cell polarization, migration and ECM-remodeling in 3D collagen matrix. FASEB J in press.

Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM. 2013. Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. J Cell Biol 202(1):163-77.

Goldmann WH, Auernheimer V, Thievessen I, Fabry B. 2013. Vinculin, cell mechanics and tumour cell invasion. Cell Biol Int 37(5):397-405.

Shin W, Fischer RS, Kanchanawong P, Kim Y, Lim J, Meyers KA, Nishimura Y, Plotnikov SV, Thievessen I, Yarar D, Sabass B, Waterman CM. 2010. A versatile, multi-color total internal reflection fluorescence and spinning disk confocal microscope system for high-resolution live cell imaging. In Live Cell Imaging. A Laboratory Manual. R.D. Goldman, J. Swedlow, D.L. Spector, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 119–138.

Manso AM, Kang SM, Plotnikov SV, Thievessen I, Oh J, Beggs HE, Ross RS. 2009. Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration. Am J Physiol Heart Circ Physiol 296(3):H627-38.

Krüger M, Moser M, Ussar S, Thievessen I, Luber CA, Forner F, Schmidt S, Zanivan S, Fässler R, Mann M. 2008. SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell 134(2):353-64.

Wang HV, Chang LW, Brixius K, Wickström SA, Montanez E, Thievessen I, Schwander M, Müller U, Bloch W, Mayer U, Fässler R. 2008. Integrin-linked kinase stabilizes myotendinous junctions and protects muscle from stress-induced damage. J Cell Biol 180(5):1037-49.

Chu H, Thievessen I, Sixt M, Lämmermann T, Waisman A, Braun A, Noegel AA, Fässler R. 2006. -Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response. Mol Cell Biol 26(5):1817-25.

Thievessen I, Wolter M, Prior A, Seifert HH, Schulz WA. 2005. Hedgehog signaling in normal urothelial cells and in urothelial carcinoma cell lines. J Cell Physiol 203(2):372-7.

Grashoff C, Thievessen I, Lorenz K, Ussar S, Fässler R. 2004. Integrin-linked kinase: integrin's mysterious partner. Curr Opin Cell Biol 16(5):565-71.

Thievessen I, Seifert HH, Swiatkowski S, Florl AR, Schulz WA. 2003. E-cadherin involved in inactivation of WNT/beta-catenin signalling in urothelial carcinoma and normal urothelial cells. Br J Cancer 88(12):1932-8.

Swiatkowski S, Seifert HH, Steinhoff C, Prior A, Thievessen I, Schliess F, Schulz WA. 2003. Activities of MAP-kinase pathways in normal uroepithelial cells and urothelial carcinoma cell lines. Exp Cell Res 282(1):48-57.

Invited Seminars and Talks

University of Zaragoza, Department of Mechanical Engineering, Zaragoza. 06/2014. Invited seminar

53rd Annual Meeting of the American Society for Cell Biology, New Orleans. 12/2013. Short talk

5th European Cell Mechanics Meeting, Obergurgl. 09/2013. Short talk

2nd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg. 09/2012. Short talk

ETH Zurich Swiss Federal Institute of Technology, Laboratory for Biologically Oriented Materials, Zurich. 06/2012. Invited seminar

4th International Symposium Interface Biology of Implants, Warnemuende. 05/2012. Short talk

51st Annual Meeting of the American Society for Cell Biology, Denver. 12/2011. Short talk

National Heart, Lung and Blood Institute, Biochemistry and Biophysics Center, Bethesda. 12/2011. Invited seminar

9th National Heart, Lung and Blood Institute Division of Intramural Research (NHLBI DIR) Scientific Retreat, Cambridge. 04/2011. Short talk

Friedrich-Alexander University Erlangen-Nuernberg, Department of Physics, Biophysics Group, Erlangen. 04/2011. Invited seminar

Max-Planck-Institute of Biochemistry, Department of Molecular Medicine, Martinsried. 09/2010. Invited seminar

National Center for Biotechnology, Department of Cellular and Molecular Biology, Madrid. 09/2010. Invited seminar

Gordon-Kenan Research Seminar “Signaling by Adhesion Receptors”, Waterville. 07/2010. Short talk

National Center for Biotechnology, 17th workshop “Advances in Molecular Biology for Young Investigators”, Madrid. 12/2009. Short talk

Poster Presentations

6th European Cell Mechanics Meeting, Barcelona, 2015

3rd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg, 2015

53rd Annual Meeting of the American Society for Cell Biology, New Orleans. 2013

2nd International Meeting of the German Society for Cell Biology on Actin dynamics, Regensburg. 2012.

51st Annual Meeting of the American Society for Cell Biology, Denver. 2011

Cell Migration Consortium meeting “Frontiers in Cell Migration & Mechanotransduction”, Bethesda. 2011

9th NHLBI DIR Scientific Retreat, Cambridge. 2011

50th Annual Meeting of the American Society for Cell Biology, Philadelphia. 2010

Gordon Research Conference “Signaling by Adhesion Receptors”, Waterville. 2010

Swedish Association for Cytoskeleton Research meeting “The Cytoskeleton in Development and Pathology”, Stockholm. 2010

8th NHLBI DIR Scientific Retreat, Baltimore. 2010

49th Annual Meeting of the American Society for Cell Biology, San Diego. 2009

National Institutes of Health Research Festival, Bethesda.2009

7th NHLBI DIR Scientific Retreat, Annapolis. 2009

European Molecular Biology Organization workshop “Signals and Mechanics in Directed Cell Migration”, Heidelberg. 2006

European Life Scientist Organisation Meeting, Dresden. 2005