Christoph Mark

Christoph Mark

Christoph Mark

 

PhD student

Room 02.076

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Conference presentations

 

Dynamics and heterogeneity in tumor cell migration - How do cells move? (Talk & Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Ben Fabry

RTG1962 Membrane Winterworkshop, Pitztal, Austria (2014)

 

Dynamics and heterogeneity in tumor cell migration (Talk & Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Lena Lautscham, Ben Fabry

5th Annual Symposium - Physics of Cancer, Leipzig, Germany (2014)

 

Uncovering the time dependent statistics of migrating tumor cells (Poster)

Christoph Mark, Claus Metzner, Julian Steinwachs, Lena Lautscham, Christoph Kämmerer, Ben Fabry

DPG-Frühjahrstagung der Sektion Kondensierte Materie, Dresden, Germany (2014)

Publications Graeme Whyte

 

Optofluidic rotation of living cells for single-cell tomography

 

gwhyte-pub-29-flowrot

 

T Kolb, S Albert, M Haug, G Whyte
Journal of Biophotonics accepted (2014)

 

Dynamic Fire Optic Spanner

 

gwhyte-DFOS-scheme

 

 

T Kolb, S Albert, M Haug, G Whyte
Lab on a Chip in press (2014)

 

Impact of heating on passive and active biomechanics of suspended cells

  gwhyte-pub-27-heating
  

 

CJ Chan, G Whyte, L Boyde, G Salbreux, J Guck
Interface Focus
accepted (2014)

 

Viscoelastic properties of differentiating cells are fate- and function-dependent

  gwhyte-pub-24-HL60-PLOSOne-2012 

 

A Ekpenyong, G Whyte, K Chalut, F Lautenschlaeger, C Fiddler, D Olin, E Chilvers, M Beil, J Guck,
PLoS ONE 7(9) (2012)

 

Mechanical environment modulates biological properties of oligodendrocyte progenitors cells

 gwhyte-pub-23-OPC-StemCellDev-2012

 

A Jagielska, A Norman, G Whyte, KJ Van Vliet, J Guck, RJM Franklin
Stem Cells and Development 21 (2012)

 

Validation and perspectives of a monolithic optical stretcher fabricated by femtosecond lasers

  gwhyte-pub-26-fsChip-BioMedOptEx-2012  

 

N Bellini, F Bragheri, R Ramponi, I Cristiani, J Guck, R Osellame, G Whyte
BioMed Opt Exp,
3, (10), 2658 (2012)

 

Comparison of Stresses on Homogeneous Spheroids in the Optical Stretcher Computed with Geometrical Optics and Generalised Lorenz-Mie Theory

gwhyte-pub-25-ComparisonStresses-AppOpt-2012  

 

L Boyde, A Ekpenyong, G Whyte, J Guck,
Applied Optics 51 (2012)

 

Generation of picoliter droplets with defined contents and concentration gradients from the separation of chemical mixtures

 gwhyte-pub-22-HPLC-AnalChem-2010
AB Theberge, G Whyte and WTS Huck
Anal Chem, 82 3449 (2010)

 

Suzuki-Miyaura coupling reactions in aqueous microdroplets with catalytically active fluorous interfaces

 Graphical abstract: Suzuki–Miyaura coupling reactions in aqueous microdroplets with catalytically active fluorous interfaces

AB Theberge, G Whyte, LM Fidalgo, R Wooton and WTS Huck
Chem. Comm, 41 6225 - 6227 (2009)

 

 

Simultaneous Determination of Gene Expression and Enzymatic Activity in Individual Bacterial Cells in Microdroplet Compartments

gwhyte-pub-20-ExpressionActivity-JACS-2009 


J Shim, LF Olguin, G Whyte, D Scott, A Babtie, C Abell, WTS Huck, G Hollfelder
JACS
131 (42) 15251 (2009)

 

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online

  gwhyte-pub-19-EmSepMS-Angewandte-2009


LM Fidalgo, G Whyte, BT Ruotolo, JLP Benesch, F Stengel, C Abell, CV Robinson and WTS Huck
Angewandte, 48, 3665 (2009)

 

Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays

 
F Courtois, LF Olguin, G Whyte, AB Theberge, WTSH Huck, C Abell, and F Hollfelder
Anal Chem, 81, (8), 3008 (2009)

 

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

gwhyte-pub-17-Static-Arrays-LoaC2009  gwhyte-pub-17-Static-Arrays-LoaC2009b
A Huebner, D Bratton, G Whyte, WTSH Huck, F Holfelder and C Abell
Lab Chip
9, 692 (2009)

 

Dynamic control of higher-order modes in hollow-core photonic crystal fibers

 gwhyte-pub-16-PCF-OptEx-2008b

 

TJ Euser, G Whyte, M Garbos, C Kaminski and P Russell
Opt Ex, 16 (22) 17972 (2008)

 

Simulated holographic three-dimensional intensity shaping of evanescent-wave fields

   
L Thomson, G Whyte, M Mazilu, and J Courtial
JOSA B, 25, 849 (2008)

 

Development of Quantitative Cell-Based Enzyme Assays in Microdroplets

 
A Huebner, LF Olguin, D Bratton, G Whyte, WTS Huck, JB Edel, C Abell and F Holifelder
Anal Chem
, 80 (10), 3890–3896 (2008)

 

From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices

 gwhyte-pub-13-EmSep-Angewandte-2008
LM Fidalgo, G Whyte, D Bratton, C Kaminski, C Abell, WTS Huck
Angewandte
, 47, 2042 (2008)

 

An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets

 gwhyte-pub-12-IVTT-ChemBioChem-2008
F Courtois, LF Olguin, G Whyte, D Bratton, WTS Huck, C Abell and F Holifelder
ChemBioChem
, 9, 439(2008)

 

Holographic assembly workstation for optical manipulation

 
G Gibson, D Carberry, G Whyte, J Leach, J Jackson, D Robert, M Miles, M Padgett,
J. Opt. A,
10 (4) 044009(2008)

 

Super-Resolution in Holographic Optical Tweezers

 


L Thomson, Y Bosseil, G Whyte, E Yao and J Courtial,
New J. Phys. 10, 023015 (2008)

 

Photorealistic visualization of imaging in canonical optical resonators

 
J Nelson, G Whyte, J Courtial,
Am. J Phys 76 (2008)

 

An optical trapped microhand for manipulating micron-sized objects

 gwhyte-pub-08-Microhand-OptEx2006
G Whyte, G Gibson, J Leach, M Padgett, D Robert and M Miles,
Opt Ex 14, 12497, (2006)

 

Optically controlled grippers for manipulating micron-sized particles

gwhyte-pub-07-Grippers-NJP2006   
G Gibson,L Barron, F Beck, G Whyte and M Padgett,
New J. Phys. 9, 14, (2007)

 

Fourier Transform in Bose-Einstein Condensates

   
G Whyte, P Öhberg, J Courtial,
New J. Phys.
8, 196, (2006)

 

Polarization and image rotation induced by a rotating dielectric rod: an optical angular momentum interpretation

 
M Padgett, G Whyte, J Girkin, A Wright, L Allen, P Öhberg, and SM Barnett
Opt Lett
31, 2205, (2006)

 

Iterative algorithms for holographic shaping of non-diffracting and self-imaging light beams

 gwhyte-pub-04-Self-imaging-beams-OptEx2005
J Courtial, G Whyte, Z Bouchal and J Wagner
Opt. Ex. 14, 2108, (2005)

 

Experimental demonstration of holographic three-dimensional light shaping using a Gerchberg-Saxton algorithm

 gwhyte-pub-03-3D-Light-Shaping-NJP2005
G Whyte and J Courtial,
New J. Phys.
7, 117, (2005)

 

Vortex sorter for Bose-Einstein condensates

   
G Whyte, J Veitch, P Öhberg and J Courtial
Phys. Rev. A.
70, 011603(R), (2004)

 

Transverse laser modes in Bose-Einstein condensates

 
G Whyte, P Öhberg and J Courtial
Phys. Rev. A. 69 053610, (2004)

Wolfgang Goldmann

Prof. Dr. Wolfgang Goldmann
Room 02.077
Biophysics group
Center for Medical Physics and Technology
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25605
fax: (+49) (0)9131-85-25601

This email address is being protected from spambots. You need JavaScript enabled to view it.

 


Research interests:

Professor Goldmann's research focuses on the protein and cell biomechanics. Specifically, it deals with the binding of membrane-bound proteins to the actin cytoskeleton and focal adhesion points as the well as with their influence on the visco/elastic/plastic behavior in cells, using biochemical and biophysical methods. In addition, Professor Goldmann is studying the function of focal adhesion proteins on cellular traction, motility, and chemical signaling in 2D and 3D environments.


Curriculum Vitae:

Professor Wolfgang H. Goldmann, PhD is a German/American citizen. He studied Medicine at Ludwig-Maximilian University in Munich and later Physical Biochemistry at the University of Bristol, England, where he received his PhD in 1990. He then moved back to Munich to take up a position under the direction of Professor Erich Sackmann at the Biophysics Department of the Technical University. From 1995 to 2004 he worked at Harvard Medical School in Boston under the auspices of Professor Amin Arnaout and Professor Don Ingber. He was a lecturer in Physical Cell Biology and Biochemistry until he left. In 2004, he spent his sabbatical as a visiting Professor at the Center for Medical Physics and Technology, Friedrich-Alexander University in Erlangen, and he is now a W2-Professor.

Ԝerner Schnеidеr

Ԝerner Schn℮id℮r

im1287ws

Electrical engineering technician

Room 02.044

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25616
fax: (+49) (0)9131-85-25601
email: ԝschn℮id℮r at biomed . uni.erlangen . de

Heike Mertin

 

Secretary

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25600;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Astrid Mainka

Astrid Mainka

Laboratory

Room 02.078
Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25615;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vera Flad

Vera Flad (Auernheimer)

DSC 0024 quer

PhD student

Room 02.074

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25607;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Project

Vinculin - mechanical force transmission in focal adhesion contacts

Mechanical coupling of the actin cytoskeleton to the extracellular matrix via the integrin-focal adhesion (FA) link is crucial for proper cellular function. The FA adaptor protein vinculin plays here a fundamental role in transmitting cellular forces. Vinculin displays an auto-inhibitory head-to-tail interaction, where it undergoes a transient conformational change from an open (active) to a closed (inactive) state. We study different vinculin mutants in mouse embryonic fibroblasts (MEFs) and measure how altered head-to-tail interactions affect cell mechanical properties.

Using the magnetic tweezer and 2D traction force microscopy, we found that vinculin in the closed state (A50I) displays lower stiffness and reduced tractions compared to vinculin in the open state (T12, E29R). We also use FRAP experiments and immunostaining to analyze the protein kinetics and focal adhesion morphology. Inserting pointmutations by site-directed mutagenesis we generate new vinculin mutants to address different questions. We focus not only on constitutively active or closed vinculin conformations but also on the influence of src-phosphorylation, PKC-phosphorylation, actin-binding or the binding to other focal adhesion proteins like p130CAS.

 

 

Curriculum Vitae

since 2010         PhD student at Biophysics Group, LPMT, Universität Erlangen-Nürnberg

2009 - 2010       Diploma thesis at Fraunhofer Institute for Biomedical Engineering, St.Ingbert

                             Thesis title: "Vergleichende Untersuchungen zur Kultivierung und Kryokonservierung von Zellen und Zellaggregaten in dreidimensionalen Kultursystemen: Neue Methoden für eine optimierte Kultur humaner embryonaler Stammzellen"

2004 - 2009       Study of biology at Julius-Maximilians-Universität Würzburg

2004                    Abitur at Gymnasium Herzogenaurach

 

 

Conferences

 Phosphorylation of vinculin alters mechanical cell properties

V. Auernheimer, L. A. Lautscham, B. Fabry, W. H. Goldmann

e-poster talk, Annual Meeting of the American Society of Cell Biology, 2013, New Orleans

 

Different aspects of vinculin activation

V. Auernheimer, L. Lautscham, B. Fabry, W.H. Goldmann

Talk, 5th European Cell Mechanics Meeting, 2013, Obergurgl

 

Influence of vinculin activation on mechanical cell properties

V. Auernheimer, L. Lautscham, S. Born, B. Hoffmann, B. Fabry, W.H. Goldmann

Poster, International Joint Meeting of the German Society for Cell Biology (DGZ)

and the German Society for Developmental Biology (GfE), 2013, Heidelberg

 

Activation and Force Transmission of Vinculin in MEFs

Auernheimer V, Janoštiak R, Brábek J, Fabry B, Goldmann WH

Talk, 4th International Symposium Interface Biology of Implants, 2012, Warnemünde

 

Conformational Switch of Vinculin: a Prerequisite for Mechanical Force Transmission

Auernheimer V, Fabry B, Goldmann WH

Poster, Annual Meeting of The American Society for Cell Biology, 2011, Denver

 

Conformational Switch of Vinculin: a Prerequisite for Mechanical Force Transmission

Auernheimer V, Fabry B, Goldmann WH

Poster, 4th European Cell Mechanics Meeting, 2011, Amsterdam

 

Head/Tail Interaction of Vinculin influences Cell Mechanical Behavior

Auernheimer V, Diez G, Fabry B, Goldmann WH

Poster, 34. Jahrestagung der Deutschen Gesellschaft für Zellbiologie, 2011, Bonn

 

 

Publications

 

Vinculin E29R mutation changes cellular mechanics

Auernheimer V., Goldmann W.H.

Biochem Biophys Res Commun. 2014 Sep 26;452(3):661-4

 

Serine phosphorylation on position 1033 of vinculin influences cell mechanical behavior

Auernheimer V., Goldmann W.H.

Biochem Biophys Res Commun. 2014 Jul 25;450(2):1095-8


Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

Lautscham L.A., Lin C.Y., Auernheimer V., Naumann C.A., Goldmann W.H. and Fabry B.
Biomaterials. 2014 Mar;35(10):3198-207


CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics

Janoŝtiak R., Brábek J., Auernheimer V., Tatárová Z., Lautscham L.A., Dey T., Gemperle J., Merkel R., Goldmann W.H., Fabry B. and Rösel D.

Cell Mol Life Sci. 2014 Feb;71(4):727-44

 

Influence of focal adhesion kinase on the mechanical behavior of cell populations

Lange J, Auernheimer V, Strissel PL, Goldmann WH

Biochem Biophys Res Commun. 2013 Jun 28;436(2):246-51

 

Vinculin, cell mechanics, and tumor cell invasion

Goldmann WH, Auernheimer V, Thievessen I, Fabry B.

Cell Biol Int. 2013 May;37(5):397-405

 

Head/Tail Intercation of Vinculin influences Cell Mechanical Behavior

Diez G, Auernheimer V, Fabry B, Goldmann WH

Biochemical and Biophysical Research Communication, 406: 229-233, 2011

 

Christian Weis

Christian Weis

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25613;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

For further information and ongoing research projects, please visit my blog.

Nadine Lang

Nadine Lang

NadineL2012

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25612;
fax: (+49) (0)9131-85-25601
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

Projects

Phd Thesis : Migration dynamics of tumor cell lines through dense 3D collagen networks


Effects of matrix stiffness and steric hindrance on cell migration in 3D

The process of cell migration in a porous 3-dimensional (3-D) connective tissue is of crucial importance for establishing proper organ function and metastasis building. Studies performed on flat 2-D surfaces revealed that cell migration strongly depends on matrix stiffness. Whether this holds true also in a 3-D porous structure is currently unclear. Here we use self-assembled collagen biopolymer networks as matrices. By varying the collagen concentration from 0.3 to 2.4 mg/ml, we achieve pore sizes between 3 to 8 µm, resembling the connective tissue matrix of organs. To increase the stiffness of the network (~2-fold) without altering collagen concentration and pore size, we added glutaraldehyde (GA) as a crosslinker. Toxic unbound aldehyde groups were removed by extensive washing with TRIS buffer. Cell migration into the matrix was studied by adding MDA-MB-231 onto the surface of collagen gels. After 3 days of culture, the cell density as a function of invasion depth was measured. Migration in the 0.3 and 0.6 mg/ml collagen gels (8 µm and 5 µm pores) was drastically enhanced after the gels were stiffened with GA. By contrast, migration in 2.4 mg/ml collagen (3 µm pores) gels was drastically reduced after the gels were stiffened with GA. Live cell imaging over 24h revealed super-diffusive and persistent migration behavior in all gels, but cells in medium-density gels migrated faster than cells in dense gels. These results demonstrate that 3-D cell migration is enhanced by higher matrix stiffness but only as long as the pore size does not fall below a critical value where it causes excessive steric hindrance.

 

Estimating the true 3D pore size distribution of biopolymer networks from directionally biased data

 

The pore size of biopolymer networks governs their mechanical and functional properties. Confocal reflection microscopy or second harmonic generation microscopy are widely used to image biopolymer networks, however, both techniques fail to resolve vertically oriented fibers. We describe how to recover the true pore size of biopolymer networks from such directionally biased data. We first determine the distribution of distances from random points in the fluid phase to the nearest fiber. This distribution follows a Rayleigh distribution, regardless of isotropy and data bias. A Rayleigh distribution is fully described by a single parameter - the characteristic pore size of the network. We show that a biased pore size can be converted to the true pore size simply by multiplication with the square root of the visible network fraction. We experimentally verify the validity of our approach by comparison with data obtained using confocal fluorescence microscopy, which represents the full structure of the network. As an important application, we investigate the pore size dependence of self-assembled collagen networks on protein concentration. We find that the pore size decreases with the square root of the collagen concentration, consistent with a total fiber length that scales linearly with the monomer concentration.

 

 

 

 

Curriculum Vitae

University

since 03/2010   

PhD Student at Lehrstuhl für physikalisch-medizinische Technik, Friedrich Alexander Universität Erlangen (FAU)

Title: Migration dynamics of tumor cell lines through dense 3D collagen networks

10/2008-01/2010      

Diploma thesis at Lehrstuhl für physikalisch-medizinische Technik, FAU

Title: A combined setup for FCS and FRAP on a budget

06/2009-09/2009 Research project at McGill University in Montreal, Canada
10/2006-10/2008 Hauptstudium Physics at FAU with focus on physics in medicine
09/2006-02/2007 Erasmuns semester at Université Lille I - Sciences et Technologies, Lille, Frankreich
10/2004-10/2006 Grundstudium Physics at Friedrich Alexander Universität Erlangen

 

School

 

09/2002-05/2004       
Sigmund-Schuckert-Gymnasium Nürnberg, Abitur
09/2001-07/2002 Helene-Lange-Gymnasium Fürth
09/2000-07/2001  Übergangsklasse am Holbein Gymnasium Augsburg
09/1997-05/2000 Dr. Max Josef Metzger Realschule Meitingen, Realschulabschluss
09/1991-07/1997 Grund-und Hauptschule Biberbach

 

Languages

 

German                        
native speaker
English fluent
French fluent (DELF/DALF - Level B2)
Spanish           advanced (Unicert II)           

 

Awards, Scholarships and Funding

09/2013  Award for best young scientist's talk at the Annual Meeting of the Society for Biomaterials
   
12/2011 Funding from Department of Gender Diversity for  Annual Meeting of American Society for Cell Biology, Denver,USA                                                                                          
03/2010                
Funding from Wilhelm and Else Heraeus foundation for DPG Frühjahrstagung, Regensburg
06/2009 Scholarship from Department of gender diversity FAU for research project in Montreal,Canada
06/2009 Funding from BaCaTech for research project in Montreal, Canada               
03/2009 Funding from Wilhelm and Else Heraeus foundation for DPG Frühjahrstagung, Dresden
09/2006-02/2007 Scholarship from Erasmus program for semester abroad in France
03/2001 Scholarship Oscar Karl Forster
05/2000 Graduated Best in Class and Region, Landkreis Augsburg

 

Conferences

First Author

 

09/2013

20th Annual Meeting 'Deutsche Gesellschaft für Biomaterialien', Erlangen

Talk and Poster„Effects of matrix stiffness and steric hindrance on 3D cell migration“

Authors: Nadine Lang, K.Skodzek, S. Hurst, A.Mainka, B.Fabry

06/2013                  

5th scientifc seminar of the northbavarian biomaterials alliance (NBBA), Würzburg  

Rapid Talk and Poster: „Effects of matrix stiffness and steric hindrance on 3D cell migration“  

Authors: Nadine Lang, K.Skodzek, S. Hurst, A.Mainka, B.Fabry

11/2012

Annual meeting 'Deutsche Gesellschaft für Biomaterialien', Hamburg  

Section Talk: „Cells respond to matrix stiffness during 3D migration through dense collagen networks“

Authors: Nadine Lang, S.Münster, K.Skodzek, A.Mainka, B.Fabry

12/2011

Annual Meeting of American Society for Cell Bioloy, Denver, Colorado,USA

Poster: „ Extracting pore size statistics of collagen gels from 3D confocal image stacks"

Authors: Nadine Lang, J.Lange, P.Krauss, C.Metzner, B.Fabry    

Poster„ Transfection can impair cell migration and mechanical cell behaviour“                  

Authors: Nadine Lang, A. Mainka, N.Bonakdar, W.Goldmann,

09/2011

4th European Cell Mechnics Meeting, Amsterdam, Netherlands

Poster: „ Transfection can impair cell migration and mechnical cell behaviour“

Authors: Nadine Lang, A. Mainka, N.Bonakdar, W.Goldmann, B.Fabry

03/2010

Annual meeting ' Deutschen physikalische Gesellschaft', Regensburg  

Poster: „Vinculin lipid anchorage infuences focal adhesion strength and turnover“ 

 Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

02/2010

 Annual Meeting of Biophysical Society, San Francisco, California, USA

Poster: „Vinculin lipid anchorage infuences focal adhesion strength and turnover“

Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

03/2009

Annual meeting ' Deutschen physikalische Gesellschaft', Dresden

Section Talk: „Vinculin lipid anchorage influences focal adhesion strength and turnover“

 

Authors: Nadine Lang,G.Diez, W.Goldmann, B.Fabry

 

 

Co-Author

11/2012                   

3rd Meeting Physics of Cancer, Leipzig

Poster:   Mechanics of Collagen Gels - What cells feel  

Authors: J.Steinwachs, C.Metzner, N.Lang, S. Münster, B.Fabry

03/2012

Annual meeting ' Deutschen physikalische Gesellschaft', Berlin

Talk: Reconstruction of Cellular Forces during Migration through Non-Linear and Non-Affine Collagen Networks

Authors: J. Steinwachs, C. Metzner, N. Lang, N. Bonakdar, S. Münster, B. Fabry 

02/2012 

Biophysical Society 56th Annual Meeting (San Diego)

Talk: Estimation of Cellular Forces during Migration through Non-Linear and Non-Affine Collagen Networks

Authors: J. Steinwachs, C. Metzner, N. Lang, N. Bonakdar, S. Münster, B. Fabry

03/2009

Annual meeting ' Deutschen physikalische Gesellschaft', Dresden

Poster: Design of a low-cost modular FCS, FRAP and Optical Tweezers setup for educational use

Authors: T.Bloem, Nadine Lang, P.Kollmannsberger, B.Fabry

 

 


Publications

 

2014

 

 

Lang N.R., Skodzek K., Hurst S., Mainka A., Steinwachs J., Schneider J, Aifanits K.  and Fabry B.

Biphasic response of cell invasion to matrix stiffness in 3-dimensional biopolymer networks

Acta Biomaterialia (accepted, November 2014)

 

 

R.Buslei,P.Strissel, C.Henke, R.Schey, Nadine Lang, M.Ruebner, C.C. Stolt, B.Fabry, R.Strick

Activation and regulation of endogenous retroviral genes in the human pituitary gland related endocrine tumors

Neuropathology and Applied Neurobiology (March  2014)

 

2013

Nadine Lang, S.Münster, C.Metzner, P.Krauss, J.Lange, S.Schürmann, K.E.Aifantis, O.Friedrich and Ben Fabry

Estimating the 3D pore size distribution of biopolymer networks from directionally biased data

Biophysical Journal (Nov 2013)

2012                              

C.Henke, M. Ruebner, F.Faschingbauer, C.C.Stolt, N. Schaefer, Nadine Lang, M.W.Beckmann, P.L.Strissel, R.Strick

Regulation of murine placentogenisis by the retroviral genes Syncetin-A, Syncetin-B and Peg10

Differentiation (accepted Feb 2013)

2012

Krauss P., Metzner C., Lange J., Nadine Lang, Fabry B.

Parameter- Free Binarization and Skeletonization of Fiber Networks from Confocal Image Stacks

PloS ONE 7:e36575, 2012  

2010           

Küpper K., Nadine Lang, Möhl C., Kirchgeßner N., Born S., Goldmann W.H., Merkel R., Hoffmann B.

Tyrosine phosphorylation of vinculin at position 1065 modi"es focal adhesion dynamics and cell tractions

Biochem Biophys Res Commun 399:560-564, 2010 



                       

 

Navid Bonakdar

Navid Bonakdar

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25604;
fax: (+49) (0)9131-85-25601; 
email: This email address is being protected from spambots. You need JavaScript enabled to view it.
website: cellmechanics.de

 

Publications

 

NEDD9 Stabilizes Focal Adhesions, Increases Binding to the Extra-Cellular Matrix and Differentially Effects 2D versus 3D Cell Migration.

Zhong J, Baquiran JB, Bonakdar N, Lees J, Ching YW, Pugacheva E, Fabry B, O'Neill GM.

PLoS One. 2012;7(4):e35058

 

3D Traction Forces in Cancer Cell Invasion

Koch TM, Münster S, Bonakdar N, Butler JP, Fabry B.

PLoS One. 2012;7(3):e33476. PMID: 22479403

 

Biomechanical characterization of a desminopathy in primary human myoblasts

Bonakdar N, Luczak J, Lautscham L, Czonstke M, Koch TM, Mainka A, Jungbauer T, Goldmann WH, Schröder R, Fabry B. Biochem Biophys Res Commun. 2012 Mar 23;419(4):703-7. PMID: 22386993

 

Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis

Legate KR, Takahashi S, Bonakdar N, Fabry B, Boettiger D, Zent R, Fässler R.

EMBO J. 2011 Nov 16;30(22):4539-53. PMID: 21926969

 

Glomerular podocytes: a study of mechanical properties and mechano-chemical signaling

Eekhoff A, Bonakdar N, Alonso JL, Hoffmann B, Goldmann WH.

Biochem Biophys Res Commun. 2011 Mar 11;406(2):229-33. PMID: 21315064

 

Filamin A is essential for active cell stiffening but not passive stiffening under external force

Kasza KE, Nakamura F, Hu S, Kollmannsberger P, Bonakdar N, Fabry B, Stossel TP, Wang N, Weitz DA.

Biophys J. 2009 May 20;96(10):4326-35. PMID: 19450503

 

Conference presentations

 

Contribution of cytoskeletal components to the nonlinear rheology of cells

Navid Bonakdar, Philip Kollmannsberger, Karen Kasza and Ben Fabry
DPG-Tagungen 2009 – Dresden

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Biophysical Society Meeting 2012 – San Diego

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
DPG-Tagungen 2012 – Berlin

 

Conference abstracts

 

Contribution of cytoskeletal components to the nonlinear rheology of cells

Navid Bonakdar, Philip Kollmannsberger, Karen Kasza and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2009 – Dresden

 

Adhesion strength of integrin-mediated bonds probed with magnetic tweezers

Navid Bonakdar, Philip Kollmannsberger, Rolf Schröder, and Ben Fabry
3rd European Meeting on Cell Mechanics 2009 – Bad Honnef

 

Mechanochemical signaling in glomerular podocytes
Anna H. Klemm, Sandra Kienle, Navid Bonakdar, Wolfgang H. Goldmann, Jose-Luis Alonso

BPS Meeting 2010 – San Francisco

 

Bond stability and loading rate-dependent properties of cell adhesion measured

with high-force magnetic tweezers

Navid Bonakdar, Claus Metzner, Geraldine O'Neill, Philip Kollmannsberger, Ben Fabry.

Biophysical Society, 55th Annual Meeting 2011 – Baltimore

 

Increase of cell stiffness in single muscle cells from patients with primary desminopathies

Navid Bonakdar, Philip Kollmannsberger, Rolf Schröder, and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2011 – Regensburg

 

Transfection with fluorescent constructs impairs the mechanical behavior of cells

Nadine Lang, Navid Bonakdar, Wolfgang Goldmann and Ben Fabry
American Society of Cell Biology, Annual Meeting 2011 - Denver

 

Direct observation of catch bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner and Ben Fabry.
American Society of Cell Biology, Annual Meeting 2011 - Denver

 

Transfection with fluorescent constructs impairs the mechanical behavior of cells

Nadine Lang, Navid Bonakdar, Wolfgang Goldmann and Ben Fabry
4th European Cell Mechanics Meeting 2011– Amsterdam

 

Increase of cell stiffness in single muscle cells from patients with primary desminopathies

Navid Bonakdar, Maja Czonke, Justyna Luczak, Lena Lautscham, Astrid Mainka, Philip Kollmannsberger, Rolf Schröder, Wolfgang Goldmann and Ben Fabry
4th European Cell Mechanics Meeting 2011– Amsterdam

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Biophysical Society, 56th Annual Meeting 2012 – San Diego

 

Estimation of Cellular Forces During Migration Through Non-Linear and Non-Affine Collagen Networks

Julian Steinwachs, Claus Metzner, Nadine Lang, Navid Bonakdar, Stefan Münster and Ben Fabry
Biophysical Society, 56th Annual Meeting 2012 – San Diego

 

Direct observation of catch-bonds in focal adhesions of living cells

Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

 

Reconstruction of Cellular Forces During Migration Through Three-Dimensional Collagen Meshworks

Julian Steinwachs, Claus Metzner, Nadine Lang, Navid Bonakdar, Stefan Münster and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

 

Cell plasticity is tightly linked to elastic stresses in the cytoskeleton

Achim Schilling, Navid Bonakdar, Michael Kuhn, Richard Gerum and Ben Fabry
Annual Meeting of the German Physical Society (DPG) 2012 - Berlin

Julian Steinwachs

Julian Steinwachs

PhD student

Room 02.073

Center for Medical Physics and Technology
Biophysics Group
Henkestrasse 91
91052 Erlangen
Germany

tel : (+49) (0)9131-85-25609;
fax: (+49) (0)9131-85-25601
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